All Hormone and Adrenal Tests

Your endocrine system controls energy, stress response, mood, growth, fertility, and bone health. All Hormone and Adrenal Tests centralizes the most useful blood and urine tests for the thyroid, adrenals, pituitary, and reproductive hormones—plus metabolic markers that influence hormone balance.

A proactive plan often starts with TSH (thyroid)A1c/glucose, and electrolytes/CMP, then adds targeted testing based on symptoms: cortisol/ACTH and cosyntropin for suspected adrenal insufficiency, late-night salivary cortisol/1-mg dex for hypercortisolism, sex-hormone panels for cycle or libido concerns, prolactin and IGF-1 for pituitary clues, and PTH/vitamin D/calcium for bone-mineral axis. These labs support screeningdiagnostic triage, and monitoring, but they do not replace a clinician’s evaluation, imaging when indicated, or urgent care for severe symptoms.

Signs, Symptoms & Related Situations

  • Energy & mood: persistent fatigue, brain fog, low mood, sleep changes

  • Weight & metabolism: weight gain/loss, belly fat, carb cravings, high triglycerides

  • Stress & blood pressure: dizziness on standing, salt craving (low BP), resistant hypertension, headache/palpitations “spells”

  • Cycles, fertility & libido: irregular or heavy periods, hot flashes, low libido/ED, infertility questions

  • Skin & hair: acne, unwanted hair growth, scalp hair thinning, dry skin

  • Growth & structure: slowed growth in children, larger hands/feet/jaw in adults (acromegaly signs)

  • Bone & mineral: fractures, low bone density, kidney stones
    Seek urgent care now for heavy bleeding with faintness, severe headache with vision changes, chest pain, shortness of breath, one-sided weakness, confusion, or shock-like symptoms.

Why These Tests Matter

What testing can do

  • Clarify causes of common symptoms (fatigue, weight change, cycle irregularity, low libido)

  • Differentiate endocrine axes (thyroid, adrenal, pituitary, reproductive) and guide the next diagnostic step

  • Monitor trends and safety once you and your clinician set a care plan

What testing cannot do

  • Provide a diagnosis or treatment plan from one number

  • Replace specialist dynamic tests or imaging (e.g., pituitary MRI, adrenal CT, pelvic ultrasound) when needed

  • Substitute for clinical judgment or emergency evaluation

What These Tests Measure (at a glance)

  • Thyroid axis: TSHFree T4 (± Free T3), TPO/Tg antibodies for autoimmune patterns. Biotin can interfere—follow hold instructions.

  • Adrenal insufficiency pathway: 8 a.m. cortisol + ACTHcosyntropin stimulationelectrolytes for sodium/potassium clues.

  • Cushing/hypercortisolism pathway: Late-night salivary cortisol (x2)1-mg dexamethasone suppression test (DST), or 24-hr urinary free cortisol (x2)ACTH to classify cause.

  • Mineralocorticoids & BP: Renin and aldosterone (ARR) for primary aldosteronism; plasma/urine metanephrines for pheochromocytoma-type spells.

  • Pituitary signals: ProlactinIGF-1 (growth hormone action), LH/FSH with testosterone or estradiolFree T4/TSH for central hypothyroidism.

  • Reproductive hormones: Estradiol, progesterone (mid-luteal), total/free testosterone, DHEA-S, SHBG, AMH, hCG (pregnancy). 17-OHP screens for nonclassic CAH when indicated.

  • Calcium-bone axis: PTHcalcium (total/ionized)vitamin D (25-OH)phosphorusmagnesium.

  • Metabolic context: A1c/fasting glucose (± insulin)lipid panel (± ApoB)CMP (AST/ALT, creatinine/eGFR, electrolytes).

Quick Build Guide

Goal Start with Add if needed
General endocrine screen TSH • A1c/Glucose • CMP/Electrolytes • Lipids Vitamin D • CBC/Iron
Fatigue + dizziness/low BP (AI concern) 8 a.m. Cortisol + ACTH • Electrolytes Cosyntropin test • Renin/Aldosterone
Cushing-type features Choose oneLNSC (x2) • 1-mg DST • UFC (x2) ACTH to classify if positive
Men’s low libido/energy Total T (×2 AM) • SHBG/Free T • LH/FSH • Prolactin • TSH A1c/Lipids • CMP
Perimenopause/menopause FSH/LH • Estradiol • TSH • Lipids • A1c Vitamin D • CBC/Iron • hCG if cycles continue
PCOS-type symptoms Total & Free T • DHEA-S • SHBG • TSH • Prolactin 17-OHP • LH/FSH • A1c/Lipids
Resistant hypertension or “spells” Electrolytes • Creatinine/eGFR Renin/Aldosterone (ARR) • Metanephrines
Bone/kidney stone concerns Calcium • PTH • 25-OH Vitamin D • Phosphorus • Mg 24-hr urine calcium (stone work-up)

How the Testing Process Works

  1. Pick your starting panel using the Quick Build Guide.

  2. Prepare for accuracy: follow fasting or timing notes (e.g., morning cortisol/testosterone; cycle-timed estradiol/progesterone). List all meds/supplements—steroidsoral estrogenbiotin, and some psych meds can affect results. Do not stop prescriptions without guidance.

  3. Get your draw/collection: blood (± saliva or 24-hr urine) at a nearby patient service center.

  4. Review results securely: combine labs with symptoms and exam; your clinician may order dynamic tests or imaging if indicated.

  5. Set a cadence: repeat selected markers to track trends and safety, especially after changes to therapy or lifestyle.

Interpreting Results (General Guidance)

  • Use patterns, not single values. Example: low Free T4 with inappropriately normal/low TSH suggests central hypothyroidism.

  • Adrenal algorithms rely on timing. Very low a.m. cortisol plus ACTH informs the need for cosyntropin testing.

  • Cortisol screening needs repetition. Two abnormal tests strengthen a Cushing diagnosis; medications and sleep can confound results.

  • Sex-hormone interpretation depends on SHBG. Calculated free T refines low-T or hyperandrogenism assessments.

  • Mineral axis is integrated. PTHcalcium, and vitamin D must be read together; ionized calcium helps when albumin is abnormal.
    Always interpret results with a qualified healthcare professional.

Choosing Panels vs. Individual Tests

  • Starter set (most adults): TSH • A1c/Glucose • CMP/Electrolytes • Lipids

  • Adrenal focus: 8 a.m. Cortisol + ACTH (± Cosyntropin) or LNSC/DST/UFC for hypercortisolism; ARR or Metanephrines for selected BP patterns

  • Reproductive focus: FSH/LH + Estradiol/Progesterone (women, cycle-timed) or Total/Free T + LH/FSH (men) with Prolactin as needed

  • Pituitary screen: Prolactin • IGF-1 • Free T4/TSH • Cortisol/ACTH • LH/FSH + Sex hormone

  • Bone/mineral: PTH • Calcium (± Ionized) • 25-OH Vitamin D • Phosphorus • Mg

FAQs

Do I need to fast?
Often for glucose/insulin and sometimes lipids. Most hormone tests don’t require fasting—follow your order.

When should I draw hormone tests?
Morning for cortisol and testosterone; day-3 for FSH/LH/E2; mid-luteal for progesterone.

Can supplements or meds affect results?
Yes—biotin can distort some immunoassays; steroids, oral estrogen, thyroid meds, antipsychotics and others matter. List everything you take.

Are saliva cortisol tests valid?
Late-night salivary cortisol is guideline-supported for Cushing screening when done at specified times.

Do labs alone diagnose PCOS or low testosterone?
No. Labs support the diagnosis and help rule out mimics; symptoms and clinical evaluation are essential.

How often should I retest?
Commonly 3–6 months after changes, then periodically once stable—your clinician will tailor timing.

Related Categories & Key Tests

  • Hormone Tests Hub

  • Endocrine Tests • Adrenal Insufficiency & Addison Disease • Cushing Syndrome • Thyroid Testing • Pituitary Disorders • Growth Hormone Tests • Men’s Hormone Tests • Women’s Hormone Tests • PCOS Tests • Parathyroid (PTH) • Metabolic Tests

  • Key Tests: TSH • Free T4/Free T3 • TPO/Tg Ab • 8 a.m. Cortisol • ACTH • Cosyntropin Test • Late-Night Salivary Cortisol • 1-mg DST • 24-hr UFC • Renin/Aldosterone (ARR) • Plasma/Urine Metanephrines • Prolactin • IGF-1 • LH/FSH • Total/Free Testosterone • Estradiol • Progesterone • DHEA-S • SHBG • 17-OHP • AMH • hCG • PTH • Calcium (Total/Ionized) • Vitamin D (25-OH) • Phosphorus • Magnesium • A1c/Glucose (± Insulin) • Lipid Panel • CMP

References

  • Endocrine Society — Clinical guidelines on adrenal insufficiency, Cushing syndrome, hypogonadism, hypopituitarism, and menopause.
  • American Thyroid Association — Thyroid testing and autoimmune thyroid disease guidance.
  • American Association of Clinical Endocrinology — Best practices across endocrine evaluation.
  • Pituitary Society — Consensus on acromegaly and prolactin disorders.
  • North American Menopause Society — Midlife assessment and hormone therapy monitoring.
  • Androgen Excess & PCOS Society — Hyperandrogenism and PCOS evaluation recommendations.
  • KDIGO — CKD-mineral bone disorder guidance (PTH, calcium, phosphorus).
  • American Diabetes Association — Standards of Care in Diabetes (A1c/glucose testing).

Available Tests & Panels

Your All Hormone and Adrenal Tests menu is pre-populated in the Ulta Lab Tests system. Use filters to:

  • build a starter panel (TSH, A1c/glucose, CMP, lipids),

  • add adrenal pathways (cortisol/ACTH, cosyntropin, LNSC/DST/UFC, ARR, metanephrines),

  • select reproductive or pituitary markers (FSH/LH, estradiol/progesterone or testosterone/SHBG, prolactin, IGF-1), and

  • include bone/mineral tests (PTH, calcium, vitamin D) as needed.
    Follow timing/fasting instructions and review results with your clinician to plan next steps and monitoring.

See the links below for lab testing for conditions influenced by hormone changes.

 

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The 17-Hydroxypregnenolone Test measures levels of this adrenal steroid precursor in the blood to help evaluate adrenal gland and steroid hormone function. Abnormal results may indicate congenital adrenal hyperplasia, enzyme deficiencies, or adrenal disorders affecting cortisol and androgen production. This test provides valuable insight into hormonal balance and supports the investigation of endocrine and metabolic health conditions.

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The 17-Hydroxyprogesterone (17-OHP) Test measures levels of 17-OHP, a hormone made by the adrenal glands, to help diagnose congenital adrenal hyperplasia (CAH) and other adrenal disorders. Elevated levels may indicate enzyme deficiencies affecting cortisol production, while low levels can suggest adrenal insufficiency. Doctors order this test for infants with ambiguous genitalia or adults with irregular periods, infertility, or abnormal androgen symptoms.

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Also Known As: 17-OHP Test, 17-OH Progesterone Test

The 21-Hydroxylase Antibody Test detects autoantibodies that target the adrenal enzyme 21-hydroxylase, often linked to autoimmune Addison’s disease and adrenal insufficiency. A positive result suggests adrenal gland damage caused by immune attack. Doctors use this blood test to confirm suspected Addison’s disease, evaluate unexplained fatigue or low cortisol, and monitor patients at risk for autoimmune polyglandular syndromes or adrenal failure.


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The Hemoglobin A1c (HbA1c) Test measures average blood glucose over 2–3 months by detecting sugar attached to hemoglobin in red blood cells. It is used to diagnose diabetes, identify prediabetes, and monitor long-term blood sugar control. Doctors rely on the HbA1c test to evaluate treatment effectiveness, guide adjustments, and assess risk for complications, making it essential for diabetes care and metabolic health screening.

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Also Known As: Hemoglobin A1c Test, HbA1c Test, Glycated Hemoglobin Test

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The Hemoglobin A1c with eAG Test measures average blood sugar levels over the past 2–3 months and provides an estimated average glucose (eAG) value for easier understanding. It is widely used to screen, diagnose, and monitor diabetes and prediabetes. Doctors use this test to evaluate long-term glucose control, adjust treatment, and reduce the risk of complications such as neuropathy, kidney disease, and heart problems linked to poor blood sugar management.

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Also Known As: Hemoglobin A1c with eAG Test, HbA1c with eAG Test, Glycated Hemoglobin with estimated Average Glucose Test

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The ACTH Test measures adrenocorticotropic hormone in blood to evaluate pituitary and adrenal gland function. Abnormal ACTH levels may indicate Cushing’s syndrome, Addison’s disease, adrenal tumors, or pituitary disorders. Doctors order this test to investigate symptoms such as fatigue, weight changes, weakness, or high blood pressure. Results provide vital insight into endocrine health, cortisol regulation, and adrenal-pituitary balance.

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Also Known As: Adrenocorticotropic Hormone Test, Corticotropin Test

The Adiponectin Test measures adiponectin, a hormone made by fat cells that helps regulate glucose levels and fatty acid breakdown. Low adiponectin is linked to insulin resistance, type 2 diabetes, obesity, and cardiovascular disease. Doctors use this test to evaluate metabolic health, assess risk for diabetes or heart disease, and monitor patients with weight-related or endocrine disorders. Results help guide prevention and treatment strategies.

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Also Known As: Fat Metabolism Test

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The Aldosterone 24 Hour Urine Test evaluates adrenal hormone output across 24 hours, offering information about blood pressure regulation, fluid balance, and electrolyte control. Elevated or low levels may suggest primary aldosteronism, adrenal tumors, or secondary hypertension. This test supports assessment of kidney function, cardiovascular health, and endocrine disorders linked to aldosterone imbalance.

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The Aldosterone and Plasma Renin Activity Ratio Test measures levels of aldosterone and renin to assess adrenal and kidney function. An elevated ratio may indicate primary aldosteronism, a common cause of secondary hypertension. Doctors use this test to evaluate patients with high blood pressure, low potassium, or suspected adrenal disorders. Results help diagnose conditions like Conn’s syndrome, guide treatment, and reduce cardiovascular risks from uncontrolled hypertension.

Also Known As: Aldosterone/Plasma Renin Activity Ratio Test, Aldosterone and Renin Activity Test

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The Aldosterone Test measures levels of aldosterone, a hormone produced by the adrenal glands that helps regulate blood pressure, sodium, and potassium balance. Abnormal levels may indicate primary aldosteronism, adrenal tumors, kidney disease, or secondary hypertension. Doctors use this blood test to investigate high blood pressure, electrolyte imbalances, or suspected adrenal disorders, helping guide diagnosis, treatment, and long-term patient management.

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Also Known As: Aldosterone Plasma Test

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The Female Anti-Mullerian Hormone (AMH) Test measures AMH levels in blood to evaluate ovarian reserve and fertility potential. AMH reflects the number of eggs remaining and helps predict response to fertility treatments such as IVF. Doctors use this test to assess reproductive lifespan, investigate irregular cycles, or diagnose conditions like polycystic ovary syndrome (PCOS). Results provide key insight into fertility, ovarian health, and reproductive planning.

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Also Known As: Anti-Mullerian Hormone Test, Mullerian-Inhibiting Hormone Test

The Male Anti-Mullerian Hormone (AMH) Test measures AMH levels in blood to assess testicular function, fertility, and hormone balance. In men, AMH is produced by Sertoli cells and plays a role in reproductive development. Doctors use this test to evaluate sperm production, investigate infertility, or assess disorders of sexual development. Results provide key insight into reproductive health, testicular activity, and overall endocrine function.

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Also Known As: Anti-Mullerian Hormone Test, Mullerian-Inhibiting Hormone Test

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The Amylase Test measures amylase enzyme levels in blood to evaluate pancreatic and digestive health. Elevated amylase may indicate pancreatitis, gallbladder disease, intestinal blockage, or salivary gland disorders, while low levels may suggest chronic pancreatitis or liver damage. Doctors order this test to investigate abdominal pain, nausea, or fever. Results provide vital insight into pancreatic function, digestive disorders, and overall metabolic health.

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Also Known As: Amy Test

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Studies show that millions of people suffering from hormonal imbalance are unaware of the condition because they do not recognize the symptoms. Hormonal imbalance is a condition that affects more than 80% of women in the United States. The imbalance in men often manifests in a decrease in testosterone levels. 

Thankfully, health researchers and practitioners conduct lab tests to help develop hormonal treatment, therapy, and management. Below is a guide to help you learn more about this condition and hormone lab tests. 

What Is a Hormonal Imbalance? 

To understand what hormone imbalance is, you should start with understanding what hormones are. The body creates hormones, tiny chemical messengers tasked with telling different organs and tissues what to do. The body produces hormones in the endocrine glands. 

Hormones are vital to how the body systems function, such as metabolism and reproduction. that is why even the slightest change in the hormonal levels in the body can result in issues that significantly impact the entire body. 

When a person is diagnosed with a hormonal imbalance, their body might have too little or too much of a particular hormone. 

What Medical Conditions Affect Hormones? 

Many different conditions can affect hormone balance, including:

  • Thyroid complications
  •  Diabetes
  • Pituitary tumors
  • Autoimmune diseases like Hashimoto's thyroiditis and Grave's disease
  • Ovarian cysts
  • PCOS

Furthermore, increased production of the growth hormone in the pituitary gland causes gigantism (acromegaly) in children. 

Risk Factors for a Hormonal Imbalance 

Studies show that lifestyle factors and increased toxin intakes are the leading causes of increased hormonal imbalance complications. Some of the other prevalent factors for this condition include: 

  • Unhealthy eating and poor diets 
  • Excess stress and depression 
  • Old age 
  • Lack of enough exercise 
  • Obesity or being overweight 
  • Consumption of toxins found in artificial food products 

Causes of a Hormonal Imbalance 

Hormone imbalance can be triggered by various things depending on the type of hormones and glands affected. Research associated such outcomes to issues like: 

  • Stress 
  • Certain medications 
  • Trauma or injury 
  • Pituitary gland tumors 
  • Eating disorders 
  • Cancerous or benign tumors 
  • Hormone replacement therapy 
  • Cancer treatments like chemotherapy 

Congenital disorders can trigger a decrease in certain hormones. Some of these disorders include complications such as: 

  • Diabetes 
  • Addison's disease 
  • Thyroiditis 
  • Hypothyroidism 
  • Thyroid nodules 
  • Hyperthyroidism 

Polycystic Ovarian Syndrome (PCOS) is the most common hormone imbalance condition in females of reproductive age. Pregnancy, breastfeeding, and menopause have also been found to trigger the imbalance in some women. 

Signs And Symptoms of a Hormonal Imbalance 

The body needs hormones to function correctly, meaning hormones are an essential part of your health. Subsequently, you can experience various symptoms depending on the increase or decrease in the production of certain hormones. Nevertheless, men and women will share signs of hormonal imbalance, which include: 

  • Infertility 
  • Irregular heart rate 
  • Unexpected weight loss or gain 
  • Joint pain, swelling or and stiffness 
  • Muscle aches, tenderness, and stiffness 
  • Frequent urination 
  • Increased thirst 
  • Sweating and increased sensitivity to heat and col 
  • Muscle weakness and fatigue 
  • Depression, anxiety, or irritability 
  • Diarrhea 
  • Constipation 

Symptoms Of Hormonal Imbalance for Females 

The signs and symptoms of hormonal imbalance in women include: 

  • Acne on the face, chest, or back 
  • Headaches 
  • Hair loss 
  • Excessive hair on the chin, face, or other body parts 
  • Night sweat 
  • Vaginal dryness 
  • Heavy, irregular periods, or missed period 
  • Painful sex 

Symptoms Of Hormonal Imbalance for Males 

Some of the common signs and symptoms of hormonal imbalance in men include: 

  • Loss of muscle mass 
  • Erectile dysfunction 
  • Difficulty with concentration 
  • Decrease in body hair 
  • Development of breast tissue 
  • Breast tenderness 
  • Loss of bone 

Children with hormonal imbalance usually experience delays in puberty. Boys have a lack of development in muscle mass or a voice that doesn't deepen. Girls will have a lack of menstrual periods and breast development. 

Diagnosis of a Hormonal Imbalance 

It is best for persons who believe they have a hormonal imbalance or notice the signs and symptoms to check with their doctor. The doctor will inquire about their medical history, ask about the symptoms they are experiencing, and do a physical exam. 

Blood tests are often done to diagnose a hormonal condition in people. The doctor can recommend further testing depending on the symptoms the person is experiencing. 

Lab Tests for Hormonal Imbalances 

A hormonal lab test often starts with a baseline blood test panel for both men and women. It will include a cortisol test that measures the cortisol (a steroid hormone produced by the adrenal gland) concentration in the blood. A DHEA exam can also be done to measure the DHEA-sulfate count in the blood. 

Checking estradiol levels is also essential. It is a test that measures the sex hormones counts in women while also assessing ovarian function. 

Men's and women's hormone test panels can include checking the luteinizing hormone or LH and FSH level or follicle-stimulating hormone. They are hormones that play a crucial role in maintaining normal reproductive system functions in men and women. For instance, assessing the testosterone levels can help determine elevated levels in females and low testosterone levels in males. 

Experts also recommend checking the thyroid-stimulating hormone to determine if the thyroid is underactive or overactive. When testing patients ' blood sugar levels, checking blood glucose and insulin levels is done, especially when ascertaining their risk of developing diabetes. 

FAQS About Hormonal Imbalances 

Q: Which specialist is the best to consult about a hormonal imbalance?  

A: It is wise to start with your family doctor. The doctor can run tests and refer you to an endocrinologist if they find or suspect you have hormone complications. 

Q: Can hormones affect our mental health? 

A: Fluctuating progesterone and estrogen hormone levels in women can lead to issues like depression and erratic moods. 

Q: What is bioidentical hormone replacement therapy? 

A: A natural hormone therapy technique uses compounds with the same structures as those the body produces to help address a hormonal imbalance problem. 

Hormone Lab Tests with Ulta Lab Tests 

Ulta Lab Tests offers tests that are highly accurate and reliable. They are an ideal option if you want precise readings to help make informed decisions about your health. With Ulta Lab Tests, you will get the following benefits: 

  • Secure and confidential results 
  • No need for health insurance 
  • Affordable pricing 
  • No need for a doctor's referral 
  • A 100% satisfaction guarantee 

Order your hormone lab tests today and get results securely online within 24 to 48 hours for most tests. 

Shop with Ulta Lab Tests and start taking charge of your health today.