Hormone Tests

This hub is for women, men, and non-binary people across life stages—anyone with concerns about energy, menstrual changes, fertility, libido, mood, hair/skin, bone health, or weight, and for those monitoring therapy (e.g., HRT/TRT) under clinician care.

Core labs include sex steroids (testosterone total/free SHBG, estradiol, progesterone), gonadotropins (LH/FSH), prolactinadrenal axis (cortisol/ACTH; late-night salivary cortisol; 24-hr urine free cortisol; 1-mg dex suppression), androgens (DHEA-S, androstenedione, 17-OHP), AMH (ovarian reserve), IGF-1 (GH axis), hCG (pregnancy), and PTH (bone/mineral).

 


What It Tests

Hormone testing helps you and your clinician:

  • Screen for common endocrine contributors to symptoms (e.g., thyroid cross-links, prolactin in cycle changes, androgens in hirsutism/acne).

  • Diagnose cycle/ovulation issues, hypogonadism, PCOS, hyperprolactinemia, adrenal disorders (Cushing’s/Addison’s), pituitary disorders, and bone/mineral imbalances.

  • Monitor therapy (TRT/HRT), recovery, and life-stage transitions (perimenopause/menopause; andropause).


Key Tests

Test Also Called (Synonyms) What It Measures Typical Prep (fasting? timing?) Specimen Turnaround Related Panels Use Type (Screening / Diagnosis / Monitoring) Timing Notes (AM, cycle day, luteal, repeat)
Testosterone (Total) SHBG TT SHBG Total T and binding (to calc Free T) Morning 7–10 a.m.; consistent routine; note meds Blood ~5–7 d Men’s Baseline, TRT Monitoring Dx/Monitoring Repeat AM if low; use LC/MS/MS for low ranges when available
Calculated Free Testosterone FT (calc) Bioavailable T (TT SHBG ± albumin) Same draw as TT/SHBG Blood ~5–7 d Men’s Baseline, TRT Monitoring Dx/Monitoring Use validated calculator; interpret with symptoms
Estradiol (E2) Sensitive E2 Estrogen status Cycle day 2–4(baseline) or per clinician; LC/MS for low ranges Blood ~3–4 d Women’s Cycle, HRT Check Dx/Monitoring Use sensitive LC/MSfor men/post-meno
Progesterone (P4) Ovulation/luteal function Mid-luteal (~7 days before menses) Blood ~1–2 d Fertility/Ovulation Dx/Monitoring Align timing to actual cycle length
LH / FSH Gonadotropins Pituitary drive; ovarian/testicular function Cycle day 2–4(women); AM(men) Blood ~1–2 d Cycle/Fertility, Hypogonadism Screen/Dx Interpret with E2/TT and clinical context
Prolactin (PRL) Hyperprolactinemia screen Morning, avoid nipple stimulation/exercise; repeat if borderline Blood ~1–2 d Pituitary Workup Screen/Dx/Monitoring Consider macroprolactin if discordant
DHEA-S Adrenal androgen AM preferred; steady vs DHEA Blood ~1–2 d PCOS/Androgen Panel Screen/Dx Useful with TT/E2/androstenedione
Androstenedione Adrenal/ovarian androgen AM; note cycle day Blood ~1–2 d PCOS/Androgen Panel Dx Pair with DHEA-S/17-OHP
17-Hydroxyprogesterone 17-OHP CAH/NC-CAH screen AM; follicular phase preferred Blood ~5–7 d PCOS/Adrenal Dx Elevated → consider ACTH stim (clinician)
Cortisol (AM serum) Diurnal peak AM 7–9 a.m.; minimize stress Blood ~1–2 d Adrenal Function Screen/Monitoring Random cortisol notfor Cushing’s screen
ACTH Pituitary ACTH AM, chilled handling Blood ~1–2 d Adrenal/Pituitary Dx/Monitoring Transport on ice; lab handling crucial
Late-Night Salivary Cortisol LNSC Cortisol at nadir Collect late night; avoid shift-work nights Saliva ~1–3 d Cushing’s Screen Dx Repeat samples increase accuracy
24-hr Urine Free Cortisol UFC Integrated cortisol output Complete 24-hrcollection; avoid missed voids Urine ~1–3 d Cushing’s Screen Dx Review meds that alter cortisol metabolism
Dexamethasone Suppression Test 1-mg DST Hypercortisolism screen Clinician-directed; dex at 11 p.m., AM cortisol draw Blood ~1–2 d Cushing’s Screen Dx Requires instructions & oversight
AMH (Anti-Müllerian Hormone) Ovarian reserve Any day; stable across cycle Blood ~5–7 d Fertility/IVF Context Screen/Monitoring Not a fertility guarantee; interpret with age/antral count
IGF-1 Somatomedin C GH axis status AM preferred; stable Blood ~1–2 d Pituitary/GH Screen/Dx/Monitoring Use age-adjusted reference range
hCG (quantitative) β-hCG Pregnancy, select tumor markers Timing per indication Blood Same–1 d Pregnancy Dx/Monitoring Serial measures for early pregnancy trends
PTH (Intact) Parathyroid hormone Calcium–PTH–Vit D axis Note Ca/Vit D supplements; fasting optional Blood ~1–2 d Bone/Mineral Dx/Monitoring Interpret with Calciumand 25-OH Vit D
(Cross-link) TSH / Free T4 / Free T3 Thyroid panel Thyroid function AM preferred; stable routine Blood ~1–2 d Thyroid Check Screen/Dx/Monitoring See Thyroid Hub for full workup

When to Test

  • Cycle changes, heavy/irregular periods, fertility planning → LH/FSH/E2 (day 2–4)P4 mid-lutealAMH any day; consider PRL and androgens for hirsutism/acne.

  • Low energy/libido or ED → Two morning TT/FT SHBG on different days; add LH/FSHPRL (clinician oversight for any therapy).

  • Suspected PCOS → TT/FT SHBGDHEA-Sandrostenedione17-OHP, ± LH/FSHPRLTSH.

  • Perimenopause/Menopause → E2FSH (contextual), TSHHRT monitoring with clinician.

  • Suspected adrenal disorder → AM cortisol ACTH, or LNSC/UFC/1-mg DST per clinician.

  • Bone/mineral issues → PTH with Calcium and 25-OH Vitamin D.

  • Pituitary symptoms (galactorrhea, headaches/visual change) → PRLIGF-1 ± axis workup.


How to Prepare

  • Timing: Prefer morning (7–10 a.m.) for TT, cortisol, ACTH. Time women’s tests by cycle phase (baseline day 2–4progesterone in mid-luteal, ~7 days before menses).

  • Fasting: Often not required; some clinicians prefer morning fasting for comparability. Follow the requisition.

  • Medications/supplements: List biotin (can interfere with some immunoassays), OCPsHRT/TRTglucocorticoidsdopamine agonists/antagoniststhyroid medsandrogens/anti-androgens.

  • Activity & collection: Avoid strenuous activity, alcohol binges, and sleep deprivation before cortisol testing; follow saliva and 24-hr urine kit instructions exactly.

  • Specimen handling: ACTH needs rapid, chilled handling; collection site will guide.


Interpreting Results

  • Testosterone: Confirm low TT with a repeat morning draw; interpret FT with SHBG; consider symptoms before any therapy.

  • Estradiol/Progesterone: Always interpret by cycle phase or menopausal status; use sensitive LC/MS assays for low ranges when relevant.

  • Prolactin: If borderline high, repeat and review medications/stress; consider macroprolactin when discordant with symptoms.

  • Cortisol: Prefer LNSCUFC, or 1-mg DST to screen for hypercortisolism; random cortisol is not helpful for Cushing’s. Shift work can confound late-night testing.

  • AMH: Indicates ovarian reserve, not egg quality or guaranteed fertility outcomes.

  • PTH/Calcium/Vitamin D: Interpret together; small changes over time matter.

  • Assay methods: LC/MS/MS improves specificity at low hormone levels; method changes can shift results.

  • No doping or illicit use: This hub does not provide guidance for performance enhancement or unsupervised hormone use.


Related Conditions

  • PCOS 

  • Menopause & Perimenopause

  • Low Testosterone / Hypogonadism

  • Adrenal Disorders (Cushing’s, Addison’s)

  • Pituitary Disorders

  • Bone & Mineral 

  • Thyroid Disorders


Bundles & Panels

  • Men’s Hormone Panel – Includes 9 tests with 13 biomarkers, such as testosterone, DHEA-S, cortisol, FSH, estradiol, offering a solid baseline assessment for male endocrine health 

  • Male Total & Free Testosterone plus SHBG – Measures both free and bound testosterone as well as SHBG, providing insights into active hormone levels and binding dynamics 

  • Female Basic Hormone Panel — Blood Test – Tests DHEA-S, estradiol, progesterone, and free & total testosterone to cover foundational hormonal balance in women 

  • Hormone-2 Essential — Women – Includes ~10 tests (15 biomarkers), expanding on basic hormones with adrenal and metabolic hormone markers for deeper insight 

  • Ulta Comprehensive Hormone Panel – The most extensive panel: 20 tests with ~108 biomarkers, covering sex hormones, thyroid, adrenal, growth factors, and full-spectrum endocrine function


FAQs

Do I need to fast for hormone labs?
Usually no. Some clinicians prefer morning fasting for consistency. Follow your test instructions.

Why do some tests require morning draws or specific cycle days?
Because many hormones follow daily rhythms (e.g., cortisol, testosterone) or cycle-phase changes(estradiol/progesterone).

Total vs free testosterone—what’s the difference?
Total T measures all testosterone; free T estimates the unbound fraction. SHBG affects free T; clinicians interpret them together.

Should men repeat testosterone before considering therapy?
Yes—two morning TT (± FT/SHBG) on different days plus symptoms and clinician evaluation.

What’s the best screen for Cushing’s?
Use late-night salivary cortisol24-hr urine free cortisol, or 1-mg overnight DST—not random cortisol.

Do birth control pills or HRT change results?
They can—share details with your clinician; timing relative to dosing may be important.

Can biotin mess up my labs?
High-dose biotin can interfere with some immunoassays. Tell the lab/clinician what you take.

Is AMH a fertility test?
AMH reflects ovarian reserve only—it doesn’t guarantee fertility or IVF success.

How often should I retest during TRT/HRT?
Per your clinician’s protocol; timing relative to dose/application is key.

Can I use these tests to enhance performance?
No. This site does not support doping or illicit hormone use.


References

  • Endocrine Society — Clinical practice guidance on hypogonadism, menopause/HRT, adrenal disorders.

  • AACE/ACE — Androgen deficiency, endocrine testing recommendations.

  • ASRM — Ovarian reserve testing (AMH), cycle timing in fertility care.

  • Pituitary Society — Hyperprolactinemia and pituitary disorders guidance.

  • NIH Office of Dietary Supplements (ODS) — Biotin interference notes; Vitamin D and related nutrients.

  • Mayo Clinic Laboratories / ARUP Consult — Test utilization, specimen handling, and method notes for endocrine assays.

Last reviewed: September 2025 by Ulta Lab Tests Medical Review Team

To get lab testing for conditions impacted by hormone shifts, see the links below.

Browse Hormone Tests Subcategories

When You Should Consider Receiving Hormone Lab Tests

Did you know more than 80% of women in the United States suffer from a hormonal imbalance, and the most common imbalance for men is a decrease in testosterone levels?

The truth is millions of people suffer from hormonal imbalances but don't always recognize the symptoms. For this reason, hormone lab tests are crucial in getting healthy and staying healthy. 

Hormone lab tests also guide hormone therapy and ongoing treatment and management of the hormonal imbalance that affects your life.

To learn more about hormonal imbalance and hormone lab tests, keep reading this guide to learn everything you need to know.

What Is a Hormonal Imbalance?

The first step to understanding a hormone imbalance is understanding hormones. Hormones are your body's tiny chemical messengers. Hormones are produced in your endocrine glands and tell your organs and tissues what to do.

Hormones are crucial to how your body functions and are responsible for your body's main processes like metabolism and reproduction. The smallest change in the number of hormones your body produces can have major effects on your entire body. 

When you're diagnosed with a hormone imbalance, you have too much or not enough of certain hormones.

Medical Conditions That Affect Hormones

Medical conditions that most affect hormones include diabetes, thyroid conditions, and pituitary tumors. 

Autoimmune diseases like Graves' Disease and Hashimoto's Thyroiditis can affect your hormone levels. Also, growth hormone problems like gigantism (acromegaly) happen in children when the pituitary gland produces too much growth hormone.

Risk Factors For a Hormonal Imbalance

Hormonal imbalances are becoming more and more common, mostly due to lifestyle factors and toxins. Risk factors for hormonal imbalance include:

  • Old age
  • Being overweight or obese
  • A poor diet
  • Not enough exercise
  • Excess stress
  • Toxins in your diet like excess sugars, chemicals, and food dyes

Causes of a Hormonal Imbalance

There are many causes of hormonal imbalance. Causes vary, depending on what glands or hormones are affected. Common causes include:

  • Hormone replacement therapy
  • Certain medications
  • Cancer treatments like chemotherapy
  • Cancerous or benign tumors
  • Pituitary gland tumors
  • Eating disorders
  • Stress
  • Trauma or injury

There are also medical conditions that lead to further hormonal imbalances like congenital disorders that cause low levels of certain hormones. Other conditions include:

  • Diabetes
  • Hyperthyroidism
  • Hypothyroidism
  • Thyroid nodules
  • Thyroiditis
  • Addison's disease

The most common cause of hormone imbalance is a polycystic ovarian syndrome (PCOS) for females of reproductive age. Other causes are menopause, pregnancy, and breastfeeding.

Signs and Symptoms of a Hormonal Imbalance

Since your hormones play a huge role in your health, there is a range of symptoms you can experience depending on which hormones are out of balance. Common signs of hormonal imbalance in both men and women include:

  • Weight gain
  • Muscle weakness and fatigue
  • Muscle aches, stiffness, and tenderness
  • Pain, swelling, and stiffness in your joints
  • A decrease or increase in your heart rate
  • Sweating and increased sensitivity to heat and cold
  • Constipation
  • Diarrhea
  • Frequent urination and increased thirst
  • Depression, anxiety, or irritability
  • Infertility

Symptoms of hormonal imbalance for females include:

  • Heavy, irregular periods, or missed periods
  • Excessive hair on your face, chin, or other body parts
  • Acne on your face, chest, or back
  • Hair loss
  • Vaginal dryness
  • Painful sex
  • Night sweats
  • Headaches

Symptoms of hormonal imbalance for males are slightly different. symptoms to watch for include:

  • Development of breast tissue
  • Breast tenderness
  • Erectile dysfunction
  • Decrease in body hair
  • Loss of muscle mass
  • Loss of bone
  • Difficulty with concentration

Children with hormonal imbalance usually experience delays in puberty. Boys have a lack of development in muscle mass or a voice that doesn't deepen. Girls will have a lack of menstrual periods and breast development.

Diagnosis of a Hormonal Imbalance

If you think you have signs and symptoms of a hormonal imbalance, it's important to make an appointment to see your doctor. 

Your doctor will ask you about your past medical history, do a physical exam and ask you about the symptoms you're experiencing. 

While there are many blood tests that can diagnose a hormone problem, your doctor will recommend testing hormones based on the symptoms you're experiencing.

Lab Tests for Hormonal Imbalances

Hormone tests start with a hormone baseline blood test panel for women and a baseline blood test panel for men.

Tests in this panel include a cortisol test to measure the amount of cortisol in your blood. Cortisol is a steroid hormone made by the adrenal gland. A DHEA levelalso measures how much DHEA-sulfate is in your blood.

An Estradiol level is another important test to measure the number of sex hormones and how your ovaries function.

The men's and women's panels include checking your follicle-stimulating hormone and luteinizing hormone or FSH and LH level. These hormones are critical to maintaining the normal function of both the male and female reproductive systems. And a testosterone level is necessary to identify low testosterone levels in males and elevated levels in females.

Another important hormone to measure is your thyroid-stimulating hormone. A thyroid-stimulating hormone level will assess if your thyroid is overactive or underactive. Finally, an insulin and blood glucose level check your blood sugar levels and risk for diabetes.

FAQS About Hormonal Imbalances

What specialist should I see about a hormonal imbalance? You should first visit your regular family doctor. If your doctor suspects you may have hormone problems, they can refer you to an endocrinologist.

How much can hormones affect my mental health? Fluctuating levels of the female hormones estrogen and progesterone can cause you to have mood swings and depression.

What is bioidentical hormone replacement therapy? Bioidentical hormone replacement therapy is a natural hormone therapy that uses compounds that have the same structures as those you naturally make in your own body.

 Hormone Lab Tests With Ulta Lab Tests

Ulta Lab Tests offers highly accurate tests, allowing you to make informed decisions about your health. Here are some amazing things to love about Ulta Lab Tests:

  • You'll always get secure and confidential results
  • Never a need for health insurance
  • No need for a physician's referral
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Order your hormone lab tests today, and your results will be provided to you securely online in 24 to 48 hours in most cases.

Take charge of your health and shop with Ulta Lab Tests today!