Low T and Testosterone Replacement Therapy (TRT)

Men receiving TRT therapy should regularly monitor their blood for adverse effects and treatment effectiveness, including testosterone measurements, complete blood count to measure hematocrit, and prostate-specific antigen testing.  Ulta Lab Tests provides reliable blood work for testosterone therapy and secure testing, so order today!    


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17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.

C-Reactive Protein Cardiac (hs CRP) Useful in predicting risk for cardiovascular disease.


A Complete Blood Count (CBC) Panel is used as a screening test for various disease states including anemia, leukemia and inflammatory processes.

A CBC blood test includes the following biomarkers: WBC, RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, Platelet count, Neutrophils, Lymphs, Monocytes, Eos, Basos, Neutrophils (Absolute), Lymphs (Absolute), Monocytes(Absolute), Eos (Absolute), Basos (Absolute), Immature Granulocytes, Immature Grans (Abs)


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Cortisol is increased in Cushing's Disease and decreased in Addison's Disease (adrenal insufficiency). Patient needs to have the specimen collected between 7 a.m.-9 a.m.


DHEA-S is the sulfated form of DHEA and is the major androgen produced by the adrenal glands. This test is used in the differential diagnosis of hirsute or virilized female patients and for the diagnosis of isolated premature adrenarche and adrenal tumors. About 10% of hirsute women with Polycystic Ovarian Syndrome (PCOS) have elevated DHEA-S but normal levels of other androgens.


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Measuring the circulating levels of estradiol is important for assessing the ovarian function and monitoring follicular development for assisted reproduction protocols. Estradiol plays an essential role throughout the human menstrual cycle. Elevated estradiol levels in females may also result from primary or secondary ovarian hyperfunction. Very high estradiol levels are found during the induction of ovulation for assisted reproduction therapy or in pregnancy. Decreased estradiol levels in females may result from either lack of ovarian synthesis (primary ovarian hypofunction and menopause) or a lesion in the hypothalamus-pituitary axis (secondary ovarian hypofunction). Elevated estradiol levels in males may be due to increased aromatization of androgens, resulting in gynecomastia.

IMPORTANT - Note this Estradiol test is not for children that have yet to start their menstrual cycle.  If this test is ordered for a child that has yet to begin their menstrual cycle Quest Diagnostics labs will substitute in Estradiol, Ultrasensitive LC/MS/MS - #30289 at an additional charge of $34



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Useful in the diagnosis of hypochromic, microcytic anemias. Decreased in iron deficiency anemia and increased in iron overload.


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FSH and LH are secreted by the anterior pituitary in response to gonadotropin-releasing hormone (GNRH) secreted by the hypothalamus. In both males and females, FSH and LH secretion is regulated by a balance of positive and negative feedback mechanisms involving the hypothalamic-pituitary axis, the reproductive organs, and the pituitary and sex steroid hormones. FSH and LH play a critical role in maintaining the normal function of the male and female reproductive systems. Abnormal FSH levels with corresponding increased or decreased levels of LH, estrogens, progesterone, and testosterone are associated with a number of pathological conditions. Increased FSH levels are associated with menopause and primary ovarian hypofunction in females and primary hypogonadism in males. Decreased levels of FSH are associated with primary ovarian hyper-function in females and primary hypergonadism in males. Normal or decreased levels of FSH are associated with polycystic ovary disease in females. In males, LH is also called interstitial cell-stimulating hormone (ICSH). Abnormal LH levels with corresponding increased or decreased levels of FSH, estrogens, progesterone, and testosterone are associated with a number of pathological conditions. Increased LH levels are associated with menopause, primary ovarian hypofunction, and polycystic ovary disease in females and primary hypo-gonadism in males. Decreased LH levels are associated with primary ovarian hyperfunction in females and primary hyper-gonadism in males.

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Measurement of GH is primarily of interest in the diagnosis and treatment of various forms of inappropriate growth hormone secretion. Growth hormone measurements in children are used in the evaluation of short stature and help differentiate low GH production from other sources of growth failure. Stimulation and suppression tests are often more meaningful than random measurements.

A Hemoglobin (Hb) A1c Blood Test evaluates the average amount of glucose in the blood. The A1c test will help determine whether you are at a higher risk of developing diabetes; to help diagnose diabetes and prediabetes; to monitor diabetes and to aid in treatment decisions.

To assist with control of blood glucose levels, the American Diabetes Association (ADA) has recommended glycated hemoglobin testing (HbA1c) twice a year for patients with stable glycemia, and quarterly for patients with poor glucose control. Interpretative ranges are based on ADA guidelines.


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Elevated levels of homocysteine are observed in patients at risk for coronary heart disease and stroke.

Insulin-like growth factor binding proteins bind IGF-I and IGF-II with high affinity but do not bind insulin. Of the six distinct IGF binding proteins structurally characterized at this time, IGFBP-3 has been shown to be the major carrier of the IGFs, transporting approximately 95% of circulating IGF-I and IGF-II. IGFBP-3 is growth hormone (GH) responsive. Thus, levels are high in cromegaly and low in hypopituitarism, and levels increase in GH-deficient children after GH administration. Thus, both assays and the ratios of IGF-I/IGFBP-2 and IGFBP-2/IGFBP-3 are useful as markers of GH action and for discriminating between growth hormone deficiency and short stature due to other etiologies in children. Other causes of short stature that result in reduced IGFBP-3 levels include poorly controlled diabetes. The IGFBP-3 assay is useful in assessing nutritional status, since IGFBP-3 decreases during both caloric and protein restriction

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Insulin-like growth factor I (IGF-I, or somatomedin C), a protein involved in stimulating somatic growth, is regulated principally by growth hormone (GH) and nutritional intake. IGF-I is transported in serum by several proteins; this helps maintain relatively high IGF-I plasma levels and minimizes fluctuations in serum IGF-I concentrations. Measuring IGF-I is useful in several growth-related disorders. Dwarfism caused by deficiency of growth hormone (hypopituitarism) results in decreased serum levels of IGF-I, while acromegaly (growth hormone excess) results in elevated levels of IGF-I. IGF-I measurements are also helpful in assessing nutritional status; levels are reduced in undernutrition and restored with a proper diet.

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This test is useful in the differential diagnosis of pituitary and gonadal insufficiency and in children with precocious puberty.

Lipid Panel includes: Total Cholesterol, HDL Cholesterol, Triglycerides, LDL-Cholesterol (calculated), Cholesterol/HDL Ratio (calculated), Non-HDL Cholesterol (calculated)Total cholesterol —this test measures all of the cholesterol in all the lipoprotein particles.High-density lipoprotein cholesterol (HDL-C) — measures the cholesterol in HDL particles; often called "good cholesterol" because it removes excess cholesterol and carries it to the liver for removal.Direct LDL - Low-density lipoprotein cholesterol (LDL-C) — calculates the cholesterol in LDL particles; often called "bad cholesterol" because it deposits excess cholesterol in walls of blood vessels, which can contribute to atherosclerosis. Usually, the amount of LDL cholesterol (LDL-C) is calculated using the results of total cholesterol, HDL-C, and triglycerides.Triglycerides — measures all the triglycerides in all the lipoprotein particles; most is in the very low-density lipoproteins (VLDL).


A lipid panel includes:Total cholesterol —this test measures all of the cholesterol in all the lipoprotein particles.High-density lipoprotein cholesterol (HDL-C) — measures the cholesterol in HDL particles; often called "good cholesterol" because it removes excess cholesterol and carries it to the liver for removal.Low-density lipoprotein cholesterol (LDL-C) — calculates the cholesterol in LDL particles; often called "bad cholesterol" because it deposits excess cholesterol in walls of blood vessels, which can contribute to atherosclerosis. Usually, the amount of LDL cholesterol (LDL-C) is calculated using the results of total cholesterol, HDL-C, and triglycerides.Triglycerides — measures all the triglycerides in all the lipoprotein particles; most is in the very low-density lipoproteins (VLDL).Very low-density lipoprotein cholesterol (VLDL-C) — calculated from triglycerides/5; this formula is based on the typical composition of VLDL particles.Non-HDL-C — calculated from total cholesterol minus HDL-C.Cholesterol/HDL ratio — calculated ratio of total cholesterol to HDL-C.


Low Testosterone [LOW T] Lab Test Review Panel -  Tested biomarkers include:

  • CBC (includes Differential and Platelets)
  • Comprehensive Metabolic Panel (CMP)
  • Cortisol, A.M.
  • DHEA Sulfate, Immunoassay
  • Estradiol
  • Ferritin
  • Hemoglobin A1c (HgbA1C)
  • Homocysteine
  • hs-CRP
  • LH
  • Lipid Panel With Ratios
  • Progesterone, Immunoassay
  • PSA Total
  • QuestAssureD™ 25-Hydroxyvitamin D (D2, D3), LC/MS/MS
  • T3 Reverse (RT3), LC/MS/MS
  • T3, Free
  • T4, Free
  • Testosterone, Total And Free And Sex Hormone Binding Globulin
  • Thyroid Peroxidase and Thyroglobulin Antibodies
  • TSH

Levels increase sharply during the luteal phase of the menstrual cycle. The level increases from 9 to 32 weeks of pregnancy.

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Elevated serum PSA concentrations have been reported in men with prostate cancer, benign prostatic hypertrophy, and inflammatory conditions of the prostate.

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In men over 50 years with total PSA between 4.0 and 10.0 ng/mL, the percent (%) free PSA gives an estimate of the probability of cancer. In these circumstances the measurement of the % free PSA may aid in avoiding unnecessary biopsies. Elevated levels of Prostate Specific Antigen (PSA) have been associated with benign and malignant prostatic disorders. Studies indicate that in men 50 years or older measurement of PSA is a useful addition to the digital rectal exam in the early detection of prostate cancer. In addition, PSA decreases to undetectable levels following complete resection of the tumor and may rise again with recurrent disease or persist with residual disease. Thus, PSA levels may be of assistance in the management of prostate cancer patients.

Testosterone, dihydrotestosterone and estrogens circulate in serum bound to Sex Hormone Binding Globulin (SHBG). SHBG concentrations are increased in pregnancy, hyperthyroidism, cirrhosis, oral estrogen administration and by certain drugs. Concentrations are decreased by testosterone, hypothyroidism, Cushings syndrome, acromegaly and obesity

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The assay may be useful in the diagnosis of nonthyroidal illness (NTI). Patients with NTI have low T3 concentrations and increased concentrations of rT3. RT3 may be useful in neonates to distinguish euthyroid sick syndrome from central hypothyroidism.

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This test is used to diagnose hyperthyroidism and to clarify thyroid status in the presence of a possible protein binding abnormality.


Are you feeling tired all the time? Have you noticed that your hair is not growing as usual? Are you struggling with your weight?

If the answer is yes, you may have low testosterone levels. This is also known as low T. It affects around 2 out of every 100 men in America. Thankfully effective therapies are available to help.

Let's take a look at testosterone replacement therapy tests, and how you can get them.

What Is the Low T and Testosterone Replacement Therapy (TRT)?

Low T is a condition in which there is a low level of the male sex hormone testosterone in the body. For a man to be diagnosed with low testosterone, it needs to be lower than 300 nanograms per deciliter (ng/dL).

Testosterone naturally exists in the body in two forms - free and testosterone that is attached to other proteins. The free testosterone circulates and having too little is linked to the symptoms mentioned at the outset. 

Testosterone replacement therapy (TRT) is hormone therapy for treating low testosterone. Testosterone can be administered in a variety of ways. These include patches, gels, injections, and even implants. Make sure you get an accurate diagnosis before starting any of these treatments.

Risk Factors for Low T and Testosterone Replacement Therapy (TRT)

There are many different reasons men develop low T. Some risk factors include:

  • Injury to the testicles
  • Chemotherapy
  • Metabolic disorders
  • Medications and illicit drug use
  • Alcohol
  • HIV/AIDS
  • Kidney failure
  • Obesity
  • Aging

If you have symptoms and know your lifestyle is not healthy, this is a good place to start.

Signs and Symptoms of Low T and Testosterone Replacement Therapy (TRT)

Symptoms of low testosterone vary from person to person. Older men are more likely to experience low testosterone than younger men. The following are common symptoms:

  • Low libido (sex drive)
  • Difficulty attaining or maintaining erections
  • Malaise
  • Feelings of depression
  • Concentration or memory problems
  • Feeling tired all the time
  • Irritability
  • Loss of muscle tone and strength

If low testosterone levels persist, there can be consequences. These include thinning of the bones, hair loss, infertility, and even breast development.

If you're experiencing any of these symptoms, it's good to get an understanding of where your baseline testosterone levels are at. 

Lab Tests for Low T and Testosterone Replacement Therapy (TRT)

The first step is to take lab tests to determine whether you are suffering from low testosterone levels. This will help your doctor decide whether or not you need treatment and the type of treatment you need.

A pre-TRT blood test will examine at least the following areas:

A complete blood count is important. It will help your doctor to see whether other factors could be contributing to your symptoms. The PSA level is important as elevated levels may indicate the presence of prostate cancer

Your doctor may advise taking your total testosterone level at two different times on two different days. Ideally, do this between 7-10 am. That's because naturally testosterone levels rise and fall in the body.

Later in the day, levels are naturally lower. So, to gain a baseline for diagnostic purposes, two tests early in the day give a clearer picture. 

Once treatment for low T has started, you will need follow-up testosterone replacement therapy lab tests. These are usually done after 4 to 6 weeks. 

The two most common lab panels ordered by practitioners are the Testosterone Replacement Therapy (TRT) Panel and the Testosterone Replacement Therapy (TRT) Plus Panel that monitor the hormonal balance, and key biomarkers and organ function that can be affected with TRT.

Frequently Asked Questions

People have a range of questions about low T and testosterone replacement therapy tests. Some of their most common are:

What are normal testosterone levels?

A normal testosterone level for a healthy man is normally considered to be more than 300 nanograms per deciliter (ng/dL). If you have a level that is lower than this, your doctor may diagnose low testosterone and recommend treatment.

What are the benefits of testosterone replacement therapy (TRT)?

The benefits of TRT include increased libido, increased muscle mass, and increased energy. Many men also report improvements in their mood and energy levels.

What are the risks of TRT?

The risks include having to take testosterone treatment long-term. If your body does not respond by naturally making more, it's possible that you will need to keep taking treatment.

What is the cost of TRT?

The cost of TRT will depend on a number of factors, including the dosage needed and whether this is covered by your insurance. Costs vary widely between $20 and $1000 per month. 

TRT should be prescribed by a physician. This ensures that you are getting the correct dose, and your condition is being monitored.

It's important to remember that testosterone replacement is not a wonder drug. You should only take it on medical advice and it's only for men who have a diagnosis of low T.

If you're experiencing a low mood, lack of energy, and a loss of interest in sex, take a look at your lifestyle. How are your diet and sleep? Do you exercise regularly? You can improve some of these issues through lifestyle changes rather than hormone therapy.

Benefits of Low T and Testosterone Replacement Therapy Tests With Ulta Lab Tests

The only way to diagnose low T is through testosterone replacement therapy tests. Never embark on treatment for low testosterone without thorough testing and sound medical advice. If you do have low T though, TRT could help you get your mojo back.

Ulta Lab Tests offers TRT testing that is highly accurate and reliable so you can make informed decisions about your health.

  • Secure and Confidential Results
  • No Insurance or Referral Needed
  • Affordable Pricing including Doctor's Order
  • 100% Satisfaction Guarantee

Order your low T and testosterone replacement therapy (TRT) lab test today. Your results will be provided to you securely and confidentially online in 24 to 48 hours for most tests.

Take charge of your health and track your progress with Ulta Lab Tests.