Our scleroderma test looks for the presence of specific autoantibodies in the blood that includes the ANA, or the antinuclear antibody test, and the serum anti-topoisomerase, also known as SCL-70. Order from Ulta Lab Tests today with results sent confidentially online.

Wondering if you should get a Scleroderma test? Find out how Scleroderma tests are helping individuals like you find answers and solutions!

Below the list of tests is a guide that explains and answers your questions on what you need to know about Scleroderma tests, along with information on Scleroderma, signs, symptoms, and diagnosis.

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Clinical Significance

Rheumatoid Arthritis Diagnostic IdentRA® Panel 2 - Early diagnosis of rheumatoid arthritis (RA), ie, diagnosis before significant joint erosion occurs, is difficult. Psoriatic arthritis can also be difficult to diagnose clinically early in the disease process, and there are no specific biomarkers. The 14-3-3η (eta) protein is an emerging biomarker for RA and erosive psoriatic arthritis diagnosis. It may play a biologic role in the joint erosive process. Blood levels appear to be elevated in patients with RA, but not in other diseases including psoriasis, osteoporosis, gout, ulcerative colitis, type 1 diabetes, systemic lupus erythematosus, Crohn disease, primary Sjögren syndrome, scleroderma, and multiple sclerosis. The 14-3-3η protein, used in conjunction with rheumatoid factor (RF) and cyclic citrullinated peptide (CCP) antibody, may improve diagnostic sensitivity in the early diagnosis of RA. It may also help differentiate those with psoriatic arthritis joint damage from those without joint damage.

Scleroderma Antibody (Scl-70)

Clinical Significance

Scl-70 antibody is present in approximately 40% of patients with progressive systemic sclerosis (PSS).

Antinuclear antibodies are associated with rheumatic diseases including Systemic Lupus Erythematous (SLE), mixed connective tissue disease, Sjogren's syndrome, scleroderma, polymyositis, CREST syndrome, and neurologic SLE. 

Reflex Information: If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge.


  • ANA Screen,IFA, with Reflex to Titer and Pattern
  • DNA (ds) Antibodies
  • Scleroderma Antibodies (SCL-70)
  • Sm and Sm/RNP Antibodies
  • Sjogren's Antibodies (SSA, SSB)

If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge - $13.00


ANAlyzeR™ ANA, IFA with Reflex Titer/Pattern, Systemic Autoimmune Panel 1


  • ANA Screen,IFA, with Reflex to Titer and Pattern
  • DNA (ds) Antibody, Crithidia IFA with Reflex to Titer
  • Chromatin (Nucleosomal) Antibody
  • Sm Antibody
  • Sm/RNP Antibody
  • RNP Antibody
  • Sjogren's Antibodies (SS-A, SS-B)
  • Scleroderma Antibody (Scl-70)
  • Jo-1 Antibody
  • Centromere B Antibody
  • Complement Component C3c and C4c
  • Cardiolipin Antibodies (IgA, IgG, IgM)
  • Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
  • Rheumatoid Factor (IgA, IgG, IgM)
  • Cyclic Citrullinated Peptide (CCP) Antibody (IgG)
  • 14.3.3 eta Protein
  • Thyroid Peroxidase Antibodies (TPO)


  • If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).
  • If the DNA (ds) Antibody Screen is positive, then DNA (ds) Antibody Titer will be performed at an additional charge (CPT code(s): 86256).


Alternative Name(s)

Expanded ANA Antibodies,Systemic Autoimmune Disorder,ANA and Expanded AI Testing,ANA and Systemic Autoimmunity,Comprehensive AI Testing,Early Systemic Autoimmune Disease,Autoimmune Disorders

Clinical Significance

Centromere B Antibody is diagnostic for the form of scleroderma known as CREST (calcinosis, Raynaud's phenomenon, esophageal immotility, sclerodactyly, and telangiectasia).

A Complete Blood Count (CBC) Panel is used as a screening test for various disease states including anemia, leukemia, and inflammatory processes.

A CBC blood test includes the following biomarkers: WBC, RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, Platelet count, Neutrophils, Lymphs, Monocytes, Eos, Basos, Neutrophils (Absolute), Lymphs (Absolute), Monocytes(Absolute), Eos (Absolute), Basos (Absolute), Immature Granulocytes, Immature Grans (Abs)

NOTE: Only measurable biomarkers will be reported.

Reflex Parameters for Manual Slide Review
  Less than  Greater Than 
WBC  1.5 x 10^3  30.0 x 10^3 
Hemoglobin  7.0 g/dL  19.0 g/dL 
Hematocrit  None  75%
Platelet  100 x 10^3  800 x 10^3 
MCV  70 fL  115 fL 
MCH  22 pg  37 pg 
MCHC  29 g/dL  36.5 g/dL 
RBC  None  8.00 x 10^6 
RDW  None  21.5
Relative Neutrophil %  1% or ABNC <500  None 
Relative Lymphocyte %  1% 70%
Relative Monocyte %  None  25%
Eosinophil  None  35%
Basophil  None  3.50%
Platelet  <75 with no flags,
>100 and <130 with platelet clump flag present,
Instrument Flags Variant lymphs, blasts,
immature neutrophils,  nRBC’s, abnormal platelets,
giant platelets, potential interference
The automated differential averages 6000+ cells. If none of the above parameters are met, the results are released without manual review.
CBC Reflex Pathway

Step 1 - The slide review is performed by qualified Laboratory staff and includes:

  • Confirmation of differential percentages
  • WBC and platelet estimates, when needed
  • Full review of RBC morphology
  • Comments for toxic changes, RBC inclusions, abnormal lymphs, and other
  • significant findings
  • If the differential percentages agree with the automated counts and no abnormal cells are seen, the automated differential is reported with appropriate comments

Step 2 - The slide review is performed by qualified Laboratory staff and includes: If any of the following are seen on the slide review, Laboratory staff will perform a manual differential:

  • Immature, abnormal, or toxic cells
  • nRBC’s
  • Disagreement with automated differential
  • Atypical/abnormal RBC morphology
  • Any RBC inclusions

Step 3 If any of the following are seen on the manual differential, a Pathologist will review the slide:

  • WBC<1,500 with abnormal cells noted
  • Blasts/immature cells, hairy cell lymphs, or megakaryocytes
  • New abnormal lymphocytes or monocytes
  • Variant or atypical lymphs >15%
  • Blood parasites
  • RBC morphology with 3+ spherocytes, RBC inclusions, suspect Hgb-C,
  • crystals, Pappenheimer bodies or bizarre morphology
  • nRBC’s

Comprehensive Metabolic Panel

Clinical Significance

Jo-1 Antibody occurs most frequently (31%) in patients with polymyositis, but has also been found in patients with dermatomyositis, and the polymyositis/scleroderma "overlap syndrome" (PM/SCL) or polymyositis/systemic lupus erythematosis "overlap syndrome" (PM/SLE).

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Elevated RF is found in collagen vascular diseases such as SLE, rheumatoid arthritis, scleroderma, Sjögren's Syndrome, and in other conditions such as leprosy, tuberculosis, syphilis, malignancy, thyroid disease and in a significant percentage of otherwise normal elderly patients.

RNP Antibody

Clinical Significance

RNP Antibodies have been associated with Mixed Connective Tissue disease.

Antibodies to Sm are highly specific for systemic lupus erythematosus (SLE) and when present are considered a marker antibody. However, these antibodies are found in only 20% of patients with SLE. RNP antibodies (also known as anti-u1 or ribonucleoprotein antibodies) are found in 45% of SLE patients but are also observed in numerous other disease states such as Sjögren's syndrome, scleroderma and polymyositis. Elevated levels of antibodies to RNP are seen in mixed connective tissue disease. In SLE, RNP antibodies have been associated with a relatively benign disease course with lower incidence of renal and central nervous system involvement. Patients may be considered positive for RNP antibodies when the RNP antibody result is significantly higher than the Sm antibody result.

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The assay may be useful in the diagnosis of nonthyroidal illness (NTI). Patients with NTI have low T3 concentrations and increased concentrations of rT3. RT3 may be useful in neonates to distinguish euthyroid sick syndrome from central hypothyroidism.

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Total T3 measurements are used to diagnose and monitor treatment of hyperthyroidism and are essential for recognizing T3 toxicosis

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This test is used to diagnose hyperthyroidism and to clarify thyroid status in the presence of a possible protein binding abnormality.

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For diagnosis of hypothyroidism and hyperthyroidism.

Note: Free T4 Index (T7) will only be calculated and reported if test code code 861 (T3 Uptake) is ordered as well.

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The free T4 are tests thelps evaluate thyroid function. The free T4 test is used to help diagnose hyperthyroidism and hypothyroidism. Free T4 is the active form of thyroxine and is usually ordered along with or following a TSH test. This helps the doctor to determine whether the thyroid hormone feedback system is functioning as it should, and the results of the tests help to distinguish between different causes of hyperthyroidism and hypothyroidism.

Thyroid Peroxidase Antibodies (TPO) and Thyroglobulin Antibodies

Measurement of thyroglobulin antibodies and thyroid peroxidase antibodies (TPO) is useful in the diagnosis and management of a variety of thyroid disorders including autoimmune thyroiditis, Hashimoto's Disease, Graves Disease and certain types of goiter.

 "IMPORTANT - Please note that Quest returns values up to 900 for the Thyroid Peroxidase Antibodies test.  If tracking requires values above 900 for the Thyroid Peroxidase Antibodies, then order test TPO Antibody Endpoint (Test Code # 15116).

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The Thyroid-stimulating Hormone (TSH) Blood Test is for differential diagnosis of primary, secondary, and tertiary hypothyroidism. The TSH test is also useful in screening for hyperthyroidism. This assay allows adjustment of exogenous thyroxine dosage in hypothyroid patients and in patients on suppressive thyroxine therapy for thyroid neoplasia.

TSI stands for thyroid stimulating immunoglobulin. TSI tells the thyroid gland to swell and release excess amounts of thyroid hormone into the blood.

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Dipstick urinalysis is important in accessing the chemical constituents in the urine and the relationship to various disease states. Microscopic examination helps to detect the presence of cells and other formed elements.

NOTE: Only measurable biomarkers will be reported.

On average, 300,000 people in the US have scleroderma. Scleroderma is an autoimmune disease with symptoms similar to many other conditions, making it difficult to diagnose.

But scleroderma is very treatable if caught early. A scleroderma test is an essential diagnostic tool in this process. If you're considering a test for scleroderma and feeling nervous, then we've got you covered.

So if you're wondering about scleroderma and scleroderma tests, then keep reading this guide to find everything you need to know.

What is Scleroderma?

Scleroderma is an autoimmune disease that affects your skin, connective tissue, and organs. Scleroderma occurs when your immune system goes a little haywire and produces too much protein collagen.

Since scleroderma happens due to your immune system, you can't catch it from anyone else. There isn't a cure for scleroderma, but there are effective treatments that can improve your symptoms and your life.

Types of Scleroderma

There are two types of scleroderma, localized and systemic scleroderma. Localized scleroderma has two main types, and the first is Morphea. With Morphea, you'll notice hardened oval patches on your skin that start reddish-purple with white centers. 

The next type of localized scleroderma is linear scleroderma. Linear scleroderma has lines and streaks of thickened skin on your arms and legs.

The next type, systemic scleroderma, is the most common. It's also referred to as generalized scleroderma, and there are two types of this.

The first is limited scleroderma which comes on slowly, first affecting the skin on your hands, feet, and face. It also causes damage to your intestines, esophagus, and lungs. Limited scleroderma is also called CREST syndrome, abbreviated to represent the common signs.

The next type of systemic scleroderma is diffuse scleroderma. Diffuse scleroderma starts quickly. The skin in your abdomen, thighs, arms, hands becomes thick, and after this affects your heart, lungs, digestive tract, and kidneys.

Risk Factors for Scleroderma

Anybody is at risk for developing scleroderma, but it mostly happens in people between 35 and 55. Women also tend to get scleroderma more often.

It's thought genetic changes, a family history of autoimmune disease and environmental triggers all play a part.

Causes of Scleroderma

Scleroderma is an autoimmune disease that affects your skin and organs, but doctors don't know the exact cause.

Symptoms and Signs of Scleroderma

Symptoms and signs of scleroderma can vary and affect different parts of your body. But you'll likely see symptoms such as:

  • Hardened skin 
  • Thick patches and appear shiny and smooth
  • Decreased blood flow to your extremities or Raynaud's Phenomenon
  • Sores on the ends of your fingers
  • Trouble swallowing
  • Painful joints
  • Joint swelling
  • Small red spots on your chest
  • Swelling of your hands and fingers
  • Heartburn
  • Diarrhea

Severe and ongoing scleroderma symptoms can cause complications like scar tissue in your lungs, loss of blood flow to your toes and fingers, and severe muscle inflammation. In some cases, you can even experience kidney, lung, or heart failure.

Treatment for Scleroderma

There is no cure or medication available to stop the overproduction of collagen in scleroderma. But many drugs can prevent complications and relieve your symptoms.

Steroid creams are often used to treat skin changes and reduce swelling. Medications to suppress the immune system, ease digestive symptoms, and prevent infections are also used.

Many people take over-the-counter pain relievers or prescription pain relievers as needed. Physical and occupational therapy is beneficial and allows people to manage pain, improve strength, and maintain their independence.

Sometimes surgery is necessary for complications of scleroderma such as amputation of severely affected limbs or organ transplants.

Diagnosing Scleroderma

If your doctor is concerned you may have scleroderma; they will start by asking you about your personal and family health history. It typically takes a series of scleroderma tests to understand the entire picture.

Scleroderma tests involve a combination of blood tests and x-rays. Doctors will also often order lung function tests and heart function tests like EKGs and echocardiograms.

A biopsy of the affected areas on your skin is often done so a specialist can closely examine the affected areas.

Scleroderma Lab Testing

No one lab test can diagnose scleroderma alone. But there are standard lab tests that help get you going in the correct diagnostic direction.

You can easily order high-quality scleroderma testing at Ulta Lab Tests. Ulta Lab Tests offers the Scleroderma Antibody test (Scl-70), which is a simple way to detect if scleroderma antibodies are present in your blood. The Scl-70 antibody occurs in about 40% of people with systemic scleroderma.

Next is the Antinuclear antibodies (ANA) test. Over 95% of people with scleroderma will test positive for ANA, with an apparent speckled pattern visible under the microscope.

The Centromere antibody (ACA) test is a test that helps detect antibodies in over 50% of people with CREST syndrome scleroderma symptoms.

General blood tests like a complete blood count and comprehensive metabolic panel are usually also ordered to check overall health.

Scleroderma Facts

Here are some important scleroderma facts to know that will help you through the diagnostic and testing process. 

  • The first description of scleroderma was in the 1700s by an Italian doctor named Carlo Curzio
  • Scleroderma is very different from one person to the other
  • It's essential to keep exercising with scleroderma
  • Eating foods that are easier to digest can help scleroderma symptoms
  • Often heartburn and Raynaud's are the first signs of scleroderma

Remember, although scleroderma isn't curable, it is very treatable!

Your Scleroderma Test

Choose Ulta Lab Tests for your scleroderma test needs. Ulta Lab Tests offers tests that are highly accurate reliable and allow you to make the best health decisions for yourself.

The best part is you don't need insurance or a referral either. Our affordable pricing includes a doctor's order, so you never have to go it alone.

We will work to make sure you're 100% satisfied and informed about your blood test results.

Most scleroderma lab test results are available within 24 to 48 hours. You can review your results confidentially and securely.

Take charge of your health, get tested and track your progress with Ulta Lab Tests.