All Stress and Fatigue Tests

All Stress and Fatigue Tests brings together the labs most often used to investigate low energy, burnout, and stress-related symptoms. Because tiredness is multifactorial, we pair HPA-axis checks (cortisol patterns, DHEA-S) with common “look-alikes,” including thyroid imbalanceanemia/iron or B12 deficiencyblood sugar issuesinflammationelectrolyte or liver/kidney problems, and selected hormone markers.

A proactive plan often starts with 8–9 a.m. serum cortisol (± ACTH) alongside TSH/free T4CBCferritin/iron/TIBCCMPA1c/fasting glucose, and vitamin D. Add late-night salivary cortisol or 24-hour urinary free cortisol when high cortisol is suspected, plus DHEA-S for longer-term adrenal output. Based on history, consider B12 ± MMAfolateCRP/ESRmagnesium, and sex hormones (e.g., testosterone in men, prolactin, or perimenopause context). These tests support screeningdiagnostic triage, and monitoring, but they do not replace a clinician’s evaluation, sleep assessment, or mental-health care.

Signs, Symptoms & Related Situations

  • Energy & sleep: persistent fatigue, afternoon slump, non-restorative sleep, early awakening, snoring or witnessed apneas

  • Stress & mood: brain fog, low motivation, irritability, anxiety under stress, low mood

  • Physical cues: lightheadedness when standing, headaches, muscle cramps, sugar/salt cravings, cold intolerance

  • Metabolic hints: central weight gain, elevated blood pressure, high triglycerides, impaired fasting glucose

  • Medication/lifestyle flags: recent or chronic steroid use, opioids, oral estrogens, shift work, overtraining, heavy alcohol

  • Urgent care now: fainting, severe weakness, vomiting with dehydration, chest pain, severe shortness of breath, confusion, high fever

Symptoms are nonspecific and should be reviewed by a qualified clinician.

Why These Tests Matter

What testing can do

  • Map cortisol patterns (morning peak, late-evening nadir) to guide next steps

  • Differentiate common mimics of fatigue (thyroid, iron/B12, glucose, inflammation, organ function)

  • Quantify risks and trends so you can measure progress after clinical or lifestyle changes

What testing cannot do

  • Diagnose “adrenal fatigue” (not a formal medical diagnosis)

  • Replace confirmatory testing for adrenal insufficiency or Cushing syndrome when indicated

  • Provide treatment or dosing advice without clinician oversight

What These Tests Measure (at a glance)

  • Cortisol & HPA-axis

    • 8–9 a.m. Serum Cortisol (± ACTH) for low-output screens

    • Late-Night Salivary Cortisol (LNSC) or 24-hr Urinary Free Cortisol (UFC) for high-output screens

    • DHEA-S for longer-term adrenal output context

    • Caveat: oral estrogens/pregnancy raise CBG, inflating total serum cortisol; free cortisol tests (LNSC/UFC) are less affected

  • Thyroid: TSH ± free T4 (± free T3 when directed)

  • Blood count & iron: CBCferritiniron/TIBCtransferrin saturation

  • Metabolic health: A1cfasting glucoseCMP (electrolytes, kidney/liver enzymes)

  • Nutrients: Vitamin B12 ± MMAfolatevitamin Dmagnesium (± RBC)

  • Inflammation: CRP (or hs-CRP steady state), ESR

  • Selected hormones (history-based): Testosterone (men—morning), prolactin, perimenopause context (clinician-directed)

Quick Build Guide

Goal / Scenario Start with Add if needed
First-line fatigue screen AM cortisol ± ACTH • TSH/free T4 • CBC • Ferritin/Iron/TIBC • CMP • A1c • Vitamin D B12 ± MMA • Folate • CRP
“Wired at night,” weight gain, HTN/glucose issues LNSC (×2) or 24-hr UFC 1-mg dex suppression test(clinician-directed)
Dizziness/low BP, steroid taper history AM cortisol ± ACTH ACTH stimulation test(clinician-directed)
Shift work/insomnia AM cortisol aligned to wake time • TSH LNSC aligned to habitual bedtime
Overtraining/athlete burnout AM cortisol • DHEA-S • CBC/CMP • Ferritin • TSH CRP • Magnesium • Vitamin D
Brain fog/neuropathy clues B12 ± MMA • Folate • TSH Iron panel • Vitamin D
Post-viral or inflammation concern CRP/ESR • CBC • CMP Targeted viral serologies(clinician-directed)

How the Testing Process Works

  1. Choose your starting bundle: match the Quick Build Guide to your symptoms and history.

  2. Prepare for accuracy: fast only if ordered (fasting glucose/lipids). Avoid vigorous exercise, nicotine, and alcohol before salivary cortisol; time AM cortisol within an hour of your usual wake time. List medicines/supplements (biotin can interfere with some assays).

  3. Provide samples: blood draw for most tests; saliva for LNSC; 24-hour urine for UFC.

  4. Review secure results: most panels post in a few days.

  5. Plan next steps: share results with your clinician to decide on confirmatory testing, sleep or mental-health evaluation, and a monitoring cadence.

Interpreting Results (General Guidance)

  • Clearly low AM cortisol with compatible symptoms → evaluate for adrenal insufficiency (confirmatory testing is clinician-directed).

  • High LNSC or UFC on repeat → consider hypercortisolism pathways (dex suppression, imaging per guidance).

  • Abnormal TSH/free T4 → thyroid imbalance can drive fatigue, mood, and weight changes.

  • Low ferritin or iron indices → iron deficiency pattern; correlate with CBC and history.

  • B12 low or MMA high → functional B12 deficiency.

  • Elevated CRP/ESR → systemic inflammation; interpret with symptoms and exam.
    Trends over time matter more than a single number; always interpret with a qualified healthcare professional.

Choosing Panels vs. Individual Tests

  • Core stress & fatigue panel: AM Cortisol ± ACTH • TSH/free T4 • CBC • Ferritin/Iron/TIBC • CMP • A1c • Vitamin D

  • HPA-axis expansion: LNSC (×2) or 24-hr UFC • DHEA-S

  • Nutrient add-ons: B12 ± MMA • Folate • Magnesium (± RBC)

  • Inflammation add-ons: CRP/hs-CRP • ESR

  • History-based hormones: Testosterone (men—AM) • Prolactin
    Choose bundled panels for efficient screening; add individual markers to answer targeted questions or monitor progress.

FAQs

Do I need to fast for these tests?
Only if your order includes fasting glucose (or lipids). Most other tests do not require fasting.

Blood, saliva, or urine—what’s the difference for cortisol?
AM serum cortisol screens low output; late-night saliva and 24-hr urine assess high output and daily pattern.

Can stress alone raise cortisol?
Yes—acute stress, poor sleep, nicotine, and intense exercise can elevate salivary cortisol. Follow prep instructions.

I’m on birth control or pregnant—does that change cortisol?
Oral estrogens/pregnancy raise CBG, increasing total serum cortisol; free cortisol tests (LNSC/UFC) are less affected.

How long after stopping steroids should I test?
Timing is individualized; recent steroid use can suppress cortisol. Ask your clinician before testing.

Will supplements affect my labs?
Some do (e.g., high-dose biotin interfering with select assays). List all products on your order and follow any washout guidance.

How often should I recheck?
Commonly 6–12 weeks after changes, then as advised by your clinician.

Related Categories & Key Tests

  • Stress & Fatigue Tests Hub

  • Adrenal Stress Tests • Thyroid Tests • Men’s Hormone Health Tests • Women’s Hormone Health Tests • Metabolism Tests • Sleep & Wellness Panels

  • Key Tests: AM Cortisol • ACTH • Late-Night Salivary Cortisol • 24-hr Urinary Free Cortisol • DHEA-S • TSH • Free T4 • CBC • Ferritin • Iron/TIBC • Transferrin Saturation • CMP • A1c • Fasting Glucose • Vitamin B12 • MMA • Folate • Vitamin D • Magnesium (± RBC) • CRP/hs-CRP • ESR • Testosterone (AM, men) • Prolactin

References

  • Endocrine Society — Guidelines on Primary Adrenal Insufficiency and Cushing Syndrome.
  • American Association of Clinical Endocrinology — Adrenal disorder and fatigue evaluation guidance.
  • American Thyroid Association — Thyroid function testing recommendations.
  • American Society of Hematology — Evaluation of anemia and iron deficiency.
  • American Diabetes Association — Standards of Care in Diabetes (A1c/glucose interpretation).
  • NIH Office of Dietary Supplements — Vitamin B12, vitamin D, iron fact sheets.
  • Clinical reviews on cortisol-binding globulin effects, salivary cortisol collection, and fatigue differentials.

Available Tests & Panels

Your All Stress and Fatigue Tests menu is pre-populated in the Ulta Lab Tests system. Start with a core stress & fatigue panel, then use filters to add HPA-axis testing (LNSC/UFC, DHEA-S), nutrient checks (B12/MMA, folate, vitamin D, magnesium), and inflammation markers (CRP/ESR). Follow any timing and collection instructions, and review results with your clinician to confirm findings and set a monitoring plan.

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Blood
Blood Draw, Phlebotomist

Blood
Blood Draw, Phlebotomist

Blood, Urine
Blood Draw, Urine Collection

Blood, Urine
Blood Draw, Urine Collection

Blood, Urine
Blood Draw, Phlebotomist, Urine Collection

Blood, Urine
Blood Draw, Phlebotomist, Urine Collection

Blood, Urine
Blood Draw, Phlebotomist, Urine Collection

The 17-Hydroxypregnenolone Test measures levels of this adrenal steroid precursor in the blood to help evaluate adrenal gland and steroid hormone function. Abnormal results may indicate congenital adrenal hyperplasia, enzyme deficiencies, or adrenal disorders affecting cortisol and androgen production. This test provides valuable insight into hormonal balance and supports the investigation of endocrine and metabolic health conditions.

Blood
Blood Draw

The 17-Hydroxyprogesterone (17-OHP) Test measures levels of 17-OHP, a hormone made by the adrenal glands, to help diagnose congenital adrenal hyperplasia (CAH) and other adrenal disorders. Elevated levels may indicate enzyme deficiencies affecting cortisol production, while low levels can suggest adrenal insufficiency. Doctors order this test for infants with ambiguous genitalia or adults with irregular periods, infertility, or abnormal androgen symptoms.

Blood
Blood Draw
Also Known As: 17-OHP Test, 17-OH Progesterone Test

The 21-Hydroxylase Antibody Test detects autoantibodies that target the adrenal enzyme 21-hydroxylase, often linked to autoimmune Addison’s disease and adrenal insufficiency. A positive result suggests adrenal gland damage caused by immune attack. Doctors use this blood test to confirm suspected Addison’s disease, evaluate unexplained fatigue or low cortisol, and monitor patients at risk for autoimmune polyglandular syndromes or adrenal failure.


Most Popular

The ACTH Test measures adrenocorticotropic hormone in blood to evaluate pituitary and adrenal gland function. Abnormal ACTH levels may indicate Cushing’s syndrome, Addison’s disease, adrenal tumors, or pituitary disorders. Doctors order this test to investigate symptoms such as fatigue, weight changes, weakness, or high blood pressure. Results provide vital insight into endocrine health, cortisol regulation, and adrenal-pituitary balance.

Blood
Blood Draw
Also Known As: Adrenocorticotropic Hormone Test, Corticotropin Test

Blood
Blood Draw, Phlebotomist

The Aldosterone 24 Hour Urine Test evaluates adrenal hormone output across 24 hours, offering information about blood pressure regulation, fluid balance, and electrolyte control. Elevated or low levels may suggest primary aldosteronism, adrenal tumors, or secondary hypertension. This test supports assessment of kidney function, cardiovascular health, and endocrine disorders linked to aldosterone imbalance.

Urine
Urine Collection

The Aldosterone and Plasma Renin Activity Ratio Test measures levels of aldosterone and renin to assess adrenal and kidney function. An elevated ratio may indicate primary aldosteronism, a common cause of secondary hypertension. Doctors use this test to evaluate patients with high blood pressure, low potassium, or suspected adrenal disorders. Results help diagnose conditions like Conn’s syndrome, guide treatment, and reduce cardiovascular risks from uncontrolled hypertension.

Also Known As: Aldosterone/Plasma Renin Activity Ratio Test, Aldosterone and Renin Activity Test

The ANA Screen IFA with Reflex to Titer and Pattern Test detects antinuclear antibodies in blood to evaluate autoimmune activity. If positive, further testing identifies antibody concentration (titer) and fluorescence pattern, helping diagnose conditions like lupus, rheumatoid arthritis, or Sjögren’s syndrome. Doctors order this test to investigate symptoms such as joint pain, fatigue, rash, or swelling and to guide treatment for autoimmune and connective tissue disorders.

Also Known As: ANA Test, Antinuclear Antibody Screen Test

The Androstenedione Test measures levels of this steroid hormone, produced by the adrenal glands and ovaries or testes, which converts into testosterone and estrogen. Abnormal results may indicate PCOS, adrenal tumors, congenital adrenal hyperplasia, or ovarian/testicular disorders. Doctors order this test for irregular periods, infertility, excess hair growth, or early puberty. Results provide key insight into hormone balance, adrenal function, and reproductive health.

Blood
Blood Draw
Also Known As: AD Test

The C-Reactive Protein (CRP) Test measures CRP levels in blood to detect inflammation in the body. Elevated CRP may indicate infections, autoimmune disorders, or chronic diseases such as arthritis, cardiovascular disease, or inflammatory bowel disease. Doctors use this test to assess acute illness, monitor treatment response, and evaluate risk for heart disease. The CRP test provides key insight into inflammation, immune health, and overall wellness.

Blood
Blood Draw
Also Known As: CRP Test, Inflammation Test

The Calcium Micronutrient Test measures calcium levels in the blood to assess nutritional status and detect imbalances. Calcium is essential for strong bones and teeth, muscle contraction, nerve signaling, and heart function. This test helps identify deficiencies, excess, or issues with absorption and metabolism, supporting diagnosis and management of bone health, hormonal balance, and overall wellness.

Patient must be 18 years of age or older.
Blood
Blood Draw
Also Known As: Ca Test, Serum Calcium Test, Calcium Blood Test

Most Popular

The Calcium Test measures calcium levels in blood to assess bone health, parathyroid function, and metabolic balance. Abnormal levels may indicate bone disease, parathyroid disorders, kidney disease, or certain cancers. Both low and high calcium can cause muscle spasms, weakness, or irregular heartbeat. Doctors order this test to monitor osteoporosis, kidney stones, or endocrine disorders. Results provide essential insight into bone, nerve, and overall metabolic health.

Blood
Blood Draw
Also Known As: Ca Test, Serum Calcium Test, Calcium Blood Test

The Heavy Metals Micronutrients Blood Test Panel measures levels of arsenic, cadmium, cobalt, lead, and mercury to assess exposure and potential toxicity. These metals, found in the environment, food, or workplaces, can affect the nervous system, kidneys, bones, and overall health. This panel helps detect harmful accumulation, guide treatment, and monitor risks from environmental or occupational exposure.

Patient must be 18 years of age or older.

The Cardio IQ™ HDL Cholesterol Test evaluates levels of high-density lipoprotein cholesterol, the “good” cholesterol that plays a critical role in heart health. Adequate HDL supports the removal of excess cholesterol from the bloodstream, reducing plaque buildup in arteries. This test provides valuable insight into cardiovascular wellness, lipid balance, and overall risk of heart disease when combined with other lipid panel results.

Blood
Blood Draw

The Cardio IQ™ Lipoprotein Fractionation Ion Mobility Test separates and measures lipoprotein particles by size and concentration using ion mobility technology. It provides a detailed profile of LDL, HDL, and other subclasses, offering a clearer assessment of cardiovascular risk than standard cholesterol testing.

Blood
Blood Draw
Also Known As: LDL Particle Testing, LDL Subclass Test, LDL Fractionations Test

The NMR Lipoprotein Fractionation Test uses nuclear magnetic resonance to measure particle size and concentration of LDL, HDL, and VLDL cholesterol. Unlike a standard lipid panel, it provides detailed information on lipoprotein subfractions linked to cardiovascular risk. Doctors use this test to assess heart disease risk, guide cholesterol management, and monitor therapy effectiveness. It offers deeper insight into lipid metabolism and cardiovascular health.

Blood
Blood Draw
Also Known As: NMR Test, LDL Particle Testing, LDL Subclass Test, LDL Fractionations Test

The Cardio IQ Vitamin D test, also known as a 25-hydroxy vitamin D test, measures the levels of total vitamin D in the blood, including both vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). It provides information about an individual's overall vitamin D status.

Blood
Blood Draw
Also Known As: Ergocalciferol Test, Vitamin D2 Test, Cholecalciferol Test, Vitamin D3 Test, Calcidiol Test, 25-hydroxyvitamin D Test, Calcifidiol Test, 25-hydroxy-vitamin D Test

The HDL Cholesterol Test measures high-density lipoprotein cholesterol, often called “good” cholesterol, which helps remove excess cholesterol from the bloodstream. Higher HDL levels are linked to a lower risk of heart disease, while low levels may increase cardiovascular risk. Doctors use this blood test as part of a lipid panel to assess heart health, evaluate risk factors, and guide lifestyle or treatment strategies for cardiovascular disease prevention.

Blood
Blood Draw
Also Known As: Good Cholesterol Test

A shocking statistic you may not know is that 55 percent of Americans experience stress during the day. That’s about one in two people! 

It’s a statistic that should get one concerned, considering what stress can do to our bodies. 

If you feel overwhelmed by stress in your day-to-day life, you should consider a stress lab test. A lab test can check various biomarkers that can help establish whether or not your well-being is taking a hit from your level of stress. 

Let’s start by learning more about stress.

What is Stress? 

Stress is the body’s reaction upon being put under pressure by internal or external stimuli. Whenever your body responds to stressful stimuli, you may have a physical, emotional, or mental response. 

It is normal for every person to deal with stress at a point in their life. It may have come about due to the loss of a loved one, dismissal from your job, or any other occurrence that made your body trigger a stress response. 

Then again, stress shouldn’t always be considered a negative thing. You could have experienced stress because you landed a new job, had your first child, or due to any other positive occurrence. Such stressors open up your mind, making you more conscious about your surroundings. 

Despite that, stress significantly affects our bodies. Whenever we’re subjected to positive or negative stressors, stress can shift the normal levels of our bodily hormones. 

Risk Factors for Stress 

We’ve already mentioned that stress is a normal part of life. As such, everyone is bound to experience it at one point in life. 

Keeping that in mind, researchers and scientists have been studying the main stressors in the patients’ lives. Below, we’ve listed the most prevalent stressors that may trigger the development of stress-related conditions: 

  • Loss of a partner 
  • Divorce 
  • Separation in marriage 
  • Incarceration 
  • Loss of a close relative 
  • Injury or sickness 
  • Marriage 
  • Dismissal from your job 
  • Marriage reunion 
  • Retirement 

If any of these has recently occurred or you’re currently going through any of these scenarios, you have to be mindful of the stress levels that are piling up. If you’re experiencing an abnormal level of stress, this could adversely affect your health. 

Ensure that you keep a keen eye on the common indicators of high stress levels. We’ll get to the details of that later on. 

Causes of Stress 

There are differences in how everyone experiences stress. When subjected to a stressor, some people may develop long-term illnesses. Some people may not experience any change even when subjected to several stressors. 

Typically, the stress you’re feeling is usually due to a significant occurrence in your life. However, what causes stress is how your body responds to such an occurrence. 

Our bodies convey a signal when subjected to stress. The signal travels throughout the body, alerting every cell of the occurrence that you’re experiencing or already went through. 

As a result, all the body systems react to the signal: your circulatory system, hormonal system, nervous system, respiratory system, and others. The response your body gives can be likened to what is experienced with the ‘flight-or-fight response.’ 

Whenever you experience stress, there’s a chain of chemical reactions that occurs. Again, how you respond to the various changes in your life is nothing out of the ordinary. 

Your body’s reaction is an adjustment to the stressor(s), and what you’re experiencing is the outcome of the changes. 

It’s possible to experience more severe symptoms and even develop sicknesses if your body doesn’t move on from this state. 

What are the signs and symptoms of Stress? 

We’ve already mentioned that stress affects all of the body’s systems. As such, numerous signs may show up whenever you’re stressed: 

  • Mood swings 
  • Feeling of being overburdened 
  • Struggling to relax 
  • Feeling lonely and/or low 
  • Keeping off family and friends 
  • Fatigue 
  • Nausea 
  • Headaches 
  • Chest pain 
  • Sleeping disorders 
  • Constant anxiety 
  • Recurrent sickness 
  • Low libido 
  • Dry mouth 
  • Constricted jaw 
  • Constant uneasiness 
  • Lapse of memory 
  • Inability to concentrate 
  • Negativity 
  • Appetite loss or gain 
  • Misjudgment 
  • Procrastination 
  • Excessive use of alcohol or drugs 

Every system in the body contributes to the symptoms of stress. This list is just the tip of the iceberg when it comes to how people feel due to stress. It is not uncommon for stress to culminate in strokes and heart attacks in some people. 

To avoid compromising your immune system or triggering other health problems, you need to suppress your stress levels. 

Diagnosing Stress 

You should see a medical professional if you’re experiencing a heightened level of stress. With their help, you can avoid being overwhelmed by stress. 

A common way to diagnose excessive stress is by evaluating your present social life. They’ll want to know if there have been recent changes or tough spells in your life. 

Another way is by running lab tests to identify any strong stress response by your body. 

Lab Tests to Observe the Effect of Stress on an Individual’s Health 

Cortisol is the most common test ordered when checking for chronic stress. Cortisol is also called the stress hormone. Your body releases cortisol when subjected to more stressors than it can handle.   

A health professional may also want to measure your testosterone and DHEA sulfate levels. Both males and females produce these sex hormones, and their levels can significantly go up when the body experiences too much stress. Such an increase can, in turn, result in other problems in the body. 

Get a Stress Lab Test with Ulta Labs Today! 

If you want to know your body’s stress level, consider a stress test from Ulta Lab Tests. We look for various biomarkers that are common indicators of stress and its effects on your health. Here’s why you should try Ulta Lab Test: 

  • Precise and dependable results that are out in 1 or 2 days for most tests 
  • Secure and confidential results 
  • No need for a doctor’s referral or insurance 
  • Affordable prices for all tests 
  • 100% guaranteed satisfaction 

Keep in mind that if the body experiences stress for too long, your well-being will likely suffer. Your body could be immunodeficient, and you could suffer acute and long-term diseases. 

Ulta Lab Tests helps you take the wheel when it comes to your health. Let us provide you with the answers you need so you can keep off stress.