Hemolytic Anemia Test

Do you feel tired all the time? Perhaps you have been sick, confused, disoriented, or weak. An increased heart rate or dark urine could be signs of a bigger problem. You may have hemolytic anemia if you've had these symptoms. 

Hemolytic anemia happens when your red blood cells are killed faster than they can be made. When you breathe in oxygen, your red blood cells carry it through your body and to your heart. 

Your blood cells are produced in your bone marrow. Your total red blood cell count will go down if your red blood cells are destroyed at a quicker pace than your bone marrow can manufacture them, resulting in hemolytic anemia.

A blood test at Ulta Lab Tests is the best way to find out if your body is breaking down your red blood cells. 

The hemolytic anemia test looks at your hemoglobin, hematocrit, and other blood components to see if you have it. A list of the anemia tests that you can order is listed below. You can get your results online in 24 to 48 hours after your samples have been collected. 

In the section below, the list of anemia tests is a guide to learn more about the advantages, results, and treatment choices for hemolytic anemia testing.


Name Matches
The detection and proper identification of hemoglobinopathies and thalassemias is an important aspect of the evaluation of patients with anemia, microcytosis and erythrocytosis.

A Complete Blood Count (CBC) Panel is used as a screening test for various disease states including anemia, leukemia, and inflammatory processes.

A CBC blood test includes the following biomarkers: WBC, RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, Platelet count, Neutrophils, Lymphs, Monocytes, Eos, Basos, Neutrophils (Absolute), Lymphs (Absolute), Monocytes(Absolute), Eos (Absolute), Basos (Absolute), Immature Granulocytes, Immature Grans (Abs)

NOTE: Only measurable biomarkers will be reported.

Reflex Parameters for Manual Slide Review
  Less than  Greater Than 
WBC  1.5 x 10^3  30.0 x 10^3 
Hemoglobin  7.0 g/dL  19.0 g/dL 
Hematocrit  None  75%
Platelet  100 x 10^3  800 x 10^3 
MCV  70 fL  115 fL 
MCH  22 pg  37 pg 
MCHC  29 g/dL  36.5 g/dL 
RBC  None  8.00 x 10^6 
RDW  None  21.5
Relative Neutrophil %  1% or ABNC <500  None 
Relative Lymphocyte %  1% 70%
Relative Monocyte %  None  25%
Eosinophil  None  35%
Basophil  None  3.50%
     
Platelet  <75 with no flags,
>100 and <130 with platelet clump flag present,
>1000 
Instrument Flags Variant lymphs, blasts,
immature neutrophils,  nRBC’s, abnormal platelets,
giant platelets, potential interference
     
The automated differential averages 6000+ cells. If none of the above parameters are met, the results are released without manual review.
CBC Reflex Pathway

Step 1 - The slide review is performed by qualified Laboratory staff and includes:

  • Confirmation of differential percentages
  • WBC and platelet estimates, when needed
  • Full review of RBC morphology
  • Comments for toxic changes, RBC inclusions, abnormal lymphs, and other
  • significant findings
  • If the differential percentages agree with the automated counts and no abnormal cells are seen, the automated differential is reported with appropriate comments

Step 2 - The slide review is performed by qualified Laboratory staff and includes: If any of the following are seen on the slide review, Laboratory staff will perform a manual differential:

  • Immature, abnormal, or toxic cells
  • nRBC’s
  • Disagreement with automated differential
  • Atypical/abnormal RBC morphology
  • Any RBC inclusions

Step 3 If any of the following are seen on the manual differential, a Pathologist will review the slide:

  • WBC<1,500 with abnormal cells noted
  • Blasts/immature cells, hairy cell lymphs, or megakaryocytes
  • New abnormal lymphocytes or monocytes
  • Variant or atypical lymphs >15%
  • Blood parasites
  • RBC morphology with 3+ spherocytes, RBC inclusions, suspect Hgb-C,
  • crystals, Pappenheimer bodies or bizarre morphology
  • nRBC’s

Before ordering this test consider The Complete Blood Count (CBC) with Differential and Platelets Blood Test (Test # 6399) which is a better value.

In Quest's internal studies of more than two thousand patient samples, no significant abnormalities were detected with manual differentials associated with test code 20253 that were not otherwise identified thru the test code 6399 CBC Reflex cascade.

This test is a CBC reflex test and it will include the components of the CBC (Includes Diff/PLT) with Smear Review based upon the test results of the following analytes if are above or below ranges as outlined in the test.

 
 
  • WBC 
  • Hemoglobin 
  • Hematocrit 
  • Platelet 
  • MCV 
  • MCH 
  • MCHC 
  • RBC 
  • RDW 
  • Relative Neutrophil % 
  • Relative Lymphocyte % 
  • Relative Monocyte % 
  • Eosinophil 
  • Basophil 
  • Platelet 

ABO Group and Rh Type

Blood typing is used to determine an individual's blood group, to establish whether a person is blood group A, B, AB, or O and whether he or she is Rh positive or Rh negative.

The Different Blood Types

There are four major blood groups and eight different blood types. Doctors call this the ABO Blood Group System.

The groups are based on whether or not you have two specific antigens -- A and B:

  • Group A has the A antigen and B antibody.
  • Group B has the B antigen and the A antibody.
  • Group AB has A and B antigens but neither A nor B antibodies.
  • Group O doesn’t have A or B antigens but has both A and B antibodies.

There’s also a third kind of antigen called the Rh factor. You either have this antigen (meaning your blood type is “Rh+” or “positive”), or you don’t (meaning your blood type is “Rh-” or “negative”). So, from the four blood groups, there are eight blood types:

  • A positive or A negative
  • B positive or B negative
  • AB positive or AB negative
  • O positive or O negative


Usual method for determining anemia. Used to calculate indices.

Most Popular
Use in evaluating erythropoietic activity.

dsDNA Antibody is detected in patients with active systemic lupus erythematosus (SLE) and approximately 20% of patients with Mixed Connective Tissue Disease.

Elevated levels of serum erythropoietin (EPO) occur in patients with anemias due to increased red cell destruction in hemolytic anemia and also in secondary polycythemias associated with impaired oxygen delivery to the tissues, impaired pulmonary oxygen exchange, abnormal hemoglobins with increased oxygen affinity, constriction of the renal vasculature, and inappropriate EPO secretion caused by certain renal and extrarenal tumors. Normal or depressed levels may occur in anemias due to increased oxygen delivery to tissues, in hypophosphatemia, and in polycythemia vera.

Comprehensive Metabolic Panel


Babesia serological testing is used to diagnose infection by the Babesia tick-borne protozoan. Infection may cause hemolytic anemia.


Most Popular
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin associated with obstructive jaundice is primarily due to the direct (conjugated) fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.

Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstructive disease

Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gallbladder obstructive disease.

Cardiolipin Antibodies (IgA, IgG, IgM)

  • Cardiolipin Antibody (IgA) 
  • Cardiolipin Antibody (IgG) 
  • Cardiolipin Antibody (IgM) 

Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly systemic lupus erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis, and primary Sjögren's syndrome.


Cardiolipin Antibodies (IgG, IgM)

  • Cardiolipin Antibody (IgG) 
  • Cardiolipin Antibody (IgM) 

Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly systemic lupus erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis, and primary Sjögren's syndrome.


Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly Systemic Lupus Erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis, and primary Sjögren's syndrome.

Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly Systemic Lupus Erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis, and primary Sjögren's syndrome.

Cardiolipin antibodies (CA) are seen in a subgroup of patients with autoimmune disorders, particularly Systemic Lupus Erythematosus (SLE), who are at risk for vascular thrombosis, thrombocytopenia, cerebral infarct and/or recurrent spontaneous abortion. Elevations of CA associated with increased risk have also been seen in idiopathic thrombocytopenic purpura, rheumatoid and psoriatic arthritis and primary Sjögren's syndrome.

Direct Antiglobulin Test (DAT) with Reflex to Anti C3 and Anti IgG

IMPORTANT - NOTE THIS IS A REFLEX TEST AND AN ADDITIONAL CHARGE OF $64 WILL OCCUR IF THE QUEST RUNS THE REFLEX TEST.

If DAT (Coombs, Direct) is positive, Anti C3d and Anti IgG will be performed at an additional charge of $64.00

Reference Range(s)

Negative

Clinical Significance

The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.

 


Assesses long term diabetic control in diabetes mellitus

See individual tests

Includes

  • CBC (includes Differential and Platelets)
  • Antibody Screen, RBC with Reflex to Identification, Titer, and Antigen Typing 
  • ABO Group and Rh Type
  • RPR (Diagnosis) with Reflex to Titer and Confirmatory Testing
  • Hepatitis B Surface Antigen with Reflex Confirmation*
  • Rubella Antibody (IgG), Immune Status
  •  
  • If Antibody Screen is positive, Antibody Identification, Titer, and Antigen Typing will be performed at an additional charge (CPT code(s): 86870, 86886, 86905).
  • If RPR screen is reactive, RPR Titer and FTA Confirmatory testing will be performed at an additional charge (CPT code(s): 86593, 86780).
  • If Hepatitis B Surface Antigen is positive, confirmatory testing based on the manufacturer's FDA approved recommendations will be performed at an additional charge (CPT code(s): 87341).
  •  

Phospholipid autoantibodies specific to phosphatidylinositol (PI), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidic acid (PA), cardiolipin (CL) and sphingomyelin are found in hematologic autoimmune diseases, especially anti-phospholipid syndrome (APS) and systemic lupus erythematosus (SLE). APS is characterized by arterial and venous thrombosis, thrombocytopenia, and recurrent fetal loss; thrombosis, thrombocytopenia and hemolytic anemia also occur in SLE and are associated with the presence of phospholipid autoantibodies

Phospholipid autoantibodies specific to phosphatidylinositol (PI), phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidic acid (PA), cardiolipin (CL) and sphingomyelin are found in hematologic autoimmune diseases, especially anti-phospholipid syndrome (APS) and systemic lupus erythematosus (SLE). APS is characterized by arterial and venous thrombosis, thrombocytopenia, and recurrent fetal loss; thrombosis, thrombocytopenia and hemolytic anemia also occur in SLE and are associated with the presence of phospholipid autoantibodies.


Have you been feeling fatigued recently? Or maybe you’ve been feeling feverish, confused, dizzy, or weak. Perhaps there are signs of a serious underlying condition, such as an increased heart rate or dark urine.

If you’ve been experiencing these symptoms, you might be suffering from hemolytic anemia.

While this can be worrying, you can, fortunately, identify the problem and treat it accordingly with lab tests for hemolytic anemia.

In this article, we’ll review everything you need to know about hemolytic anemia and how getting a lab test can help you.

Finally, you can find out if you have hemolytic anemia and get the treatment you need to be healthy. Read on to learn more.

What Is Hemolytic Anemia?

Hemolytic anemia is a condition in which your red blood cells are being destroyed faster than they can be made. Your red blood cells have an essential mission, to carry oxygen from your lungs through your body and toward your heart.

Your bone marrow creates your red blood cells. However, when your red blood cells are destroyed at a faster rate than your bone marrow can produce, your total red blood cell count will go down, resulting in hemolytic anemia.

When this occurs, you are experiencing hemolytic anemia.

About Hemolytic Anemia

There are two types of hemolytic anemia. The first is extrinsic hemolytic. When this occurs, it develops in a variety of ways. It can be caused by autoimmune hemolytic anemia or by your spleen trapping and destroying healthy red blood cells. Other causes can include:

  • Tumors
  • Infection
  • Side effects of medication
  • Autoimmune disorders
  • Lymphoma
  • Leukemia

The other type of hemolytic anemia is intrinsic hemolytic. When this occurs, the blood cells your body produces aren’t functioning properly. These conditions are usually inherited. For example, thalassemia or sickle cell disease can cause you to have abnormal hemoglobin.

Risk Factors for Hemolytic Anemia

The risk of hemolytic anemia can be higher for people with atypical pneumonia and viral infections such as mononucleosis. Additionally, certain medicines can make it more likely for people to get it, as can certain cancers. These cancers include lymphoma and leukemia.

Anyone with a family history of hemolytic diseases or an autoimmune collagen-vascular disease, like systemic lupus erythematosus, is also at risk.

Causes of Hemolytic Anemia

Causes of hemolytic anemia include intravascular hemolysis, paroxysmal nocturnal hemoglobinuria, hereditary spherocytosis, and cold agglutinin disease. Any disease or condition that destroys red blood cells can cause hemolytic anemia, such as:

  • Intravascular hemolysis
  • Paroxysmal nocturnal hemoglobinuria
  • Hereditary spherocytosis
  • Cold agglutinin disease

Other causes also include:

  • Infectious hepatitis
  • An enlarged spleen
  • Typhoid fever
  • The Epstein-Barr virus
  • The E. coli toxin
  • HELP syndrome
  • Wiskot Aldritch syndrome

Specific medications that can cause drug-induced hemolytic anemia include certain antibiotics, acetaminophen (more commonly known as Tylenol), and ibuprofen.

They also include chlorpromazine, procainamide, interferon-alpha, rifampin, and quinidine.

Additionally, if you got a blood transfusion and were given the wrong blood type, this can cause severe hemolytic anemia.

Signs and Symptoms of Hemolytic Anemia

Signs and symptoms of hemolytic anemia include:

  • Fatigue
  • Paleness of the skin
  • Confusion
  • Fever
  • Dizziness
  • Lightheadedness
  • Weakness
  • Inability to do physical activity
  • A heart murmur
  • An enlarged spleen
  • An increased heart rate

Other signs and symptoms include an enlarged spleen and jaundice, where your skin and the whites of your eyes go yellow.

Lab Tests for Hemolytic Anemia

There are several hemolytic anemia tests available. In addition to the physical exam, there are lab tests that include blood tests such as a reticulocyte count test, a peripheral smear (also called the blood smear), and a Coombs’ test.

They also include liver function, hemoglobin, and bilirubin tests. Let’s review all of these in detail now.

Reticulocyte Count Test

When you take this hemolytic anemia lab test, the hemolysis labs practitioner will determine the percentage or number of reticulocytes in your blood. This will help them determine whether you have an issue with conditions like bone marrow disorders or anemia.

Peripheral Smear

The peripheral smear, or blood smear, can be used to determine many blood-related issues, such as problems with platelets (white blood cells) and red blood cells. They’ll be able to confirm if your red blood cell count is low and whether you have sickle cell disease.

They’ll get a step closer to understanding if you have hemolytic anemia and what the underlying cause is.

The Coombs’ Test

A Direct Antiglobulin Test, (DAT) also known as Coombs’ test, can determine if antibodies act against your red blood cells’ surfaces. If these antibodies are present, this will confirm that you have hemolytic anemia.

Liver Function Test

This test measures the bilirubin, liver enzymes, and protein levels present in your blood. With these measurements, the lab practitioner will be able to find out whether the condition causing your symptoms is hemolytic anemia.

Hemoglobin Test

With this test, you’ll get an indirect reflection of the red blood cell amount you have circulating within your blood. Specifically, it measures the protein that carries oxygen within the red blood cells going through your body.

Bilirubin Test

Finally, there’s the bilirubin test. This test will measure bilirubin levels in your blood. Bilirubin is the blood cell hemoglobin that has been broken down and processed by your liver. This will help determine if you have hemolytic anemia.

Frequently Asked Questions About Hemolytic Anemia and Lab Testing for Hemolytic Anemia

There are several frequently asked questions that you may be asking yourself about hemolytic anemia. We’ll review them now so that you can get all the questions answered before deciding whether or not you want to take a test.

What Type of Doctor Does Hemolytic Anemia Treatment?

Usually, your family physician or your child’s pediatrician will diagnose hemolytic anemia. If necessary, they’ll refer you to a hematologist. This is a doctor who specializes in conditions such as hemolytic anemia.

What Complications Can Hemolytic Anemia Lead To?

If left untreated, hemolytic anemia can lead to kidney failure, which is dangerous, or to gallstones, downright unpleasant. Another worrying complication it can lead to is heart failure. For this reason, it’s important to get a test for this condition.

Can I Learn More About Hemolytic Disease of the Newborn?

If the mother’s blood group or Rh-factors do not match with those of her newborn, this can cause hemolytic disease of the newborn. When this happens, the newborn can end up experiencing edema or newborn jaundice.

Treatment requires the baby with blood transfusions and providing the mother with the RhoGAM injection.

Benefits of Hemolytic Anemia Lab Testing With Ulta Lab Tests

Ulta Lab Tests offers tests that are highly accurate and reliable so that you can make informed decisions about your health. Benefits include secure and confidential results, no insurance or referral needed, affordable price including the doctor’s order, and more.

Secure and Confidential Results

Your results are secure and confidential when you book a lab test for hemolytic anemia with Ulta Lab Tests. Results will be sent directly to you and no one else, so you can feel comfortable knowing you’re taking care of your health without having to worry about anyone else seeing your results.

No Insurance or Referral Needed

One of the reasons you may be hesitant to get a test is because you don’t have insurance. You might not be able to afford a doctor to give you a referral. We cut through all this red tape and make tests available to you.

Affordable Pricing Including the Doctor’s Order

We also make our pricing affordable to get the test you need without paying an exorbitant amount. This includes the Doctor’s Order, which saves you more money than you’d have to pay for the traditional route.

100% Satisfaction Guarantee

Finally, we provide a 100% satisfaction guarantee. This can give you the assurance you need when taking a test with us.

Want to Order Your Hemolytic Anemia Test?

Now that you’ve read about the benefits of testing for hemolytic anemia, results, and treatment options, you might be thinking of ordering a test. In this case, you should look no further than Ulta Lab Tests, as we have the most affordable, secure, and thorough testing available.

Order your Hemolytic Anemia lab test today, and your results will be provided to you securely and confidentially online in 24 to 48 hours for most tests.

Take charge of your health and track your progress with Ulta Lab Tests.