Estradiol, FSH and Testosterone Free and Total

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The following is a list of what is included in the item above. Click the test(s) below to view what biomarkers are measured along with an explanation of what the biomarker is measuring.


Estradiol (estradiol-17 beta, E2) is part of an estrogen that is a group of steroids that regulate the menstrual cycle and function as the main female sex hormones. Estrogens are responsible for the development of female sex organs and secondary sex characteristics and are tied to the menstrual cycle and pregnancy. They are considered the main sex hormones in women and are present in small quantities in men. Estradiol (E2) is the predominant form of estrogen and is produced primarily in the ovaries with additional amounts produced by the adrenal glands in women and in the testes and adrenal glands in men. Estradiol levels are used in evaluating ovarian function. Estradiol levels are increased in cases of early (precocious) puberty in girls and gynecomastia in men. Its main use has been in the differential diagnosis of amenorrhea – for example, to determine whether the cause is menopause, pregnancy, or a medical problem. In assisted reproductive technology (ART), serial measurements are used to monitor follicle development in the ovary in the days prior to in vitro fertilization. Estradiol is also sometimes used to monitor menopausal hormone replacement therapy.

Also known as: Follicle Stimulating Hormone, FSH Follicle Stimulating Hormone


Also known as: Testosterone Free Dialysis and Total LCMSMS

Free Testosterone

In many cases, measurement of total testosterone provides the doctor with adequate information. However, in certain cases, for example when the level of SHBG is abnormal, a test for free or bioavailable testosterone may be performed as it may more accurately reflect the presence of a medical condition.


A testosterone test measures the amount of the male hormone, testosterone, in the blood. Both men and women produce this hormone. In males, the testicles produce most of the testosterone in the body. Levels are most often checked to evaluate signs of low testosterone: In boys -- early or late puberty and in men -- impotence, low level of sexual interest, infertility, thinning of the bones In females, the ovaries produce most of the testosterone and levels are most often checked to evaluate signs of higher testosterone levels, such as: decreased breast size, excess hair growth, increased size of the clitoris. irregular or absent menstrual periods and male-pattern baldness or hair thinning.
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The Estradiol, FSH and Testosterone Free and Total panel contains 3 tests with 4 biomarkers.

Brief Description: The Estradiol, FSH (Follicle Stimulating Hormone), and Testosterone Free and Total panel is a comprehensive hormonal assessment designed to evaluate the balance and levels of key sex hormones in the body. This panel includes tests for Estradiol, a form of estrogen that plays a crucial role in the reproductive system and overall health; Follicle Stimulating Hormone (FSH), which is pivotal in the regulation of the reproductive process; and Testosterone Free and Total, assessing both the bound and unbound testosterone levels, which are vital for various bodily functions beyond the reproductive system.

Collection Method: Blood Draw

Specimen Type: Serum

Test Preparation: No preparation required

When and Why the Panel May Be Ordered

This panel is often ordered when symptoms suggest hormonal imbalances or disorders related to the reproductive system, such as infertility, irregular menstrual cycles, or symptoms of menopause in women, and symptoms of low testosterone in men, such as reduced libido, fatigue, and muscle weakness. It's also utilized in monitoring hormone replacement therapy and assessing the function of reproductive organs.

What the Panel Checks For

  • Estradiol: This test measures the amount of estradiol, a form of estrogen, which is significant for reproductive and sexual function and helps maintain healthy bones and cardiovascular health.
  • Follicle Stimulating Hormone (FSH): FSH is crucial for the development of follicles in ovaries in women and spermatogenesis in men. This test helps evaluate fertility issues, menstrual irregularities, and pituitary function.
  • Testosterone Free and Total: This test measures both the total testosterone and the testosterone that is not bound to proteins in the blood. Testosterone affects sexual features and development, muscle mass, and mood.

Conditions and Diseases Detected by the Panel

This hormonal panel can help in diagnosing and managing a range of conditions:

  • Reproductive Health Issues: Such as polycystic ovary syndrome (PCOS) in women, marked by elevated testosterone levels, or ovarian failure, indicated by high FSH levels.
  • Menopause: Characterized by decreased estradiol levels and increased FSH levels.
  • Male Hypogonadism: Low testosterone levels can indicate this condition, leading to reduced muscle mass, fatigue, and erectile dysfunction.
  • Pituitary Disorders: Abnormal FSH levels may suggest pituitary gland disorders affecting hormonal regulation.

Using the Panel Results in Clinical Practice

Healthcare professionals utilize the results from this panel to:

  • Diagnose Hormonal Imbalances: Identifying the underlying causes of symptoms related to sexual development, reproductive health, and metabolic functions.
  • Guide Treatment Decisions: For conditions like menopause, PCOS, or male hypogonadism, hormone replacement therapy or other treatments may be adjusted based on hormone levels.
  • Monitor Therapy Efficacy: In patients undergoing hormone replacement or treatments affecting hormonal balance, this panel aids in monitoring treatment progress and making necessary adjustments.

The Estradiol, FSH, and Testosterone Free and Total panel offers a comprehensive look at key sex hormones, providing valuable insights into reproductive health, hormonal balance, and underlying medical conditions. By understanding the interplay of these hormones, healthcare providers can tailor treatment strategies to individual needs, enhancing patient care and quality of life in those affected by hormonal imbalances.

We advise having your results reviewed by a licensed medical healthcare professional for proper interpretation of your results.

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