Estradiol and Testosterone Total

The Estradiol and Testosterone Total panel contains 2 tests with 2 biomarkers.

Brief Description: The Estradiol and Testosterone Total Test is a diagnostic blood test that measures the levels of two key hormones in the body: estradiol and total testosterone. These hormones play crucial roles in various physiological processes, particularly in reproductive and sexual health, making this test important for assessing hormone balance.

This test is not intended for patients under the age of 18 years old. For patients under 18 years old, order Estradiol, Ultrasensitive LC/MS/MS #30289. If Estradiol #4021 is ordered for a patient under 18, the lab will automatically change the code to and charge for Estradiol, Ultrasensitive LC/MS/MS #30289 at an additional charge of $34.

Collection Method: Blood Draw

Specimen Type: Serum

Test Preparation: No preparation required

Note: For any patients for whom low estradiol levels are anticipated (e.g. males, pre-pubertal children and hypogonadal/post-menopausal females), the Estradiol, Ultrasensitive, LC/MS/MS #30289 assay is recommended.

When and Why the Estradiol and Testosterone Total Test May Be Ordered

Timing of the Test: Healthcare providers may order the Estradiol and Testosterone Total Test for several reasons:

  1. Reproductive Health Assessment: This test is often ordered as part of a comprehensive evaluation of reproductive health, especially in individuals experiencing symptoms or issues related to fertility, menstruation, or sexual function.

  2. Menopausal or Perimenopausal Evaluation: Estradiol levels can fluctuate significantly during menopause and perimenopause. Monitoring estradiol levels helps assess hormonal changes during this phase of life.

  3. Evaluation of Hormone Replacement Therapy (HRT): For individuals undergoing hormone replacement therapy, this test is used to monitor hormone levels and ensure that hormone replacement doses are appropriate.

  4. Assessment of Symptoms: When individuals experience symptoms such as irregular menstrual cycles, hot flashes, mood changes, or sexual dysfunction, this test can help identify potential hormonal imbalances as contributing factors.

  5. Evaluation of Androgen-Related Disorders: In cases where symptoms suggest androgen excess or deficiency, measuring testosterone levels is crucial. This includes conditions such as polycystic ovary syndrome (PCOS) and hypogonadism.

What the Estradiol and Testosterone Total Test Checks For

The Estradiol and Testosterone Total Test assesses the following hormonal markers:

  • Estradiol: Estradiol is the primary form of estrogen in the body and is responsible for regulating the menstrual cycle, promoting breast development, maintaining bone density, and influencing mood and cognition. Abnormal estradiol levels may indicate issues with ovarian function, menopause, or certain health conditions.

  • Testosterone Total: Total testosterone includes both free testosterone (unbound and biologically active) and testosterone bound to proteins. Testosterone plays a key role in male and female sexual health, muscle mass, bone density, and overall well-being. Abnormal levels can be indicative of hormonal imbalances or underlying medical conditions.

How Health Care Providers Use the Results of the Estradiol and Testosterone Total Test

Healthcare providers use the results of the Estradiol and Testosterone Total Test to:

  1. Evaluate Reproductive Health: For individuals experiencing fertility issues, irregular menstruation, or menopausal symptoms, the test helps identify hormonal imbalances that may contribute to these problems.

  2. Assess Hormone Replacement Therapy: In cases where hormone replacement therapy is prescribed, the test helps providers monitor hormone levels to ensure that treatment is effective and safe.

  3. Diagnose Androgen-Related Conditions: Abnormal testosterone levels can be indicative of conditions such as PCOS or hypogonadism, guiding the diagnosis and management of these disorders.

  4. Personalize Treatment: The results inform healthcare providers when hormone-related interventions or treatments are necessary, such as hormone replacement therapy or medications to address hormonal imbalances.

  5. Monitor Overall Health: Estradiol and testosterone levels can also provide insights into bone health, cardiovascular risk, and mood disorders, allowing for comprehensive health assessment.

In summary, the Estradiol and Testosterone Total Test is a valuable tool for evaluating reproductive and sexual health, monitoring hormone replacement therapy, and diagnosing and managing conditions related to hormonal imbalances. It assists healthcare providers in tailoring treatment plans and optimizing overall well-being.

We advise having your results reviewed by a licensed medical healthcare professional for proper interpretation of your results.

The following is a list of what is included in the item above. Click the test(s) below to view what biomarkers are measured along with an explanation of what the biomarker is measuring.


Estradiol (estradiol-17 beta, E2) is part of an estrogen that is a group of steroids that regulate the menstrual cycle and function as the main female sex hormones. Estrogens are responsible for the development of female sex organs and secondary sex characteristics and are tied to the menstrual cycle and pregnancy. They are considered the main sex hormones in women and are present in small quantities in men. Estradiol (E2) is the predominant form of estrogen and is produced primarily in the ovaries with additional amounts produced by the adrenal glands in women and in the testes and adrenal glands in men. Estradiol levels are used in evaluating ovarian function. Estradiol levels are increased in cases of early (precocious) puberty in girls and gynecomastia in men. Its main use has been in the differential diagnosis of amenorrhea – for example, to determine whether the cause is menopause, pregnancy, or a medical problem. In assisted reproductive technology (ART), serial measurements are used to monitor follicle development in the ovary in the days prior to in vitro fertilization. Estradiol is also sometimes used to monitor menopausal hormone replacement therapy.

Also known as: Testosterone, Testosterone Total LCMSMS, Testosterone, Total


A testosterone test measures the amount of the male hormone, testosterone, in the blood. Both men and women produce this hormone. In males, the testicles produce most of the testosterone in the body. Levels are most often checked to evaluate signs of low testosterone: In boys -- early or late puberty and in men -- impotence, low level of sexual interest, infertility, thinning of the bones In females, the ovaries produce most of the testosterone and levels are most often checked to evaluate signs of higher testosterone levels, such as: decreased breast size, excess hair growth, increased size of the clitoris. irregular or absent menstrual periods and male-pattern baldness or hair thinning.
*Process times are an estimate and are not guaranteed. The lab may need additional time due to weather, holidays, confirmation/repeat testing, or equipment maintenance.

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